Core--Transgenic mouse/ES cell

转基因小鼠/胚胎干细胞共享资源由Microinjection和胚胎干细胞实验室组成。总之，这两个组成部分提供了七种不同的服务，以促进转基因和基因敲除小鼠的产生、维持或储存。在过去的三年半里，这个资源已经服务了35个不同的癌症中心研究者（总共50个不同的研究者）。显微注射实验室已经培育出273只转基因创始小鼠（通过DNA显微注射）和508只嵌合小鼠（通过胚胎干细胞显微注射）。1995年7月开始运作的胚胎干细胞实验室已经帮助培育了至少九种不同的基因靶向小鼠。另外还发展了四项新的服务，现在向调查人员提供。这些措施包括对已建立的细胞系进行再分化、辅助生殖、胚胎冷冻保存，以及为想要进行自己的显微注射实验的研究人员租用显微注射设备。这一资源已成功地满足了对其服务的要求，随着生物医学研究中对生殖系改变小鼠的需求不断增长，预计将继续提供现有和新的服务。

The transgenic Mouse/ES Cell Shared Resource consists of both the Microinjection and ES Cell Laboratories. Together, these two components provide seven different services that facilitate the generation, maintenance, or storage of transgenic and gene knock- out mice. Over the past three and a half years this resource has served 35 different Cancer Center investigators (of a total of 50 different investigators). The Microinjection Laboratory has generated over 273 transgenic founder mice (made via DNA microinjections) and 508 chimeric mice (made via ES cell microinjections). The ES Cell laboratory, which began operation in July, 1995, has assisted in the generation of at least nine different gene-targeted mice. Four other new services have been developed and are now provided to investigators. These include rederivation of established lines, assisted reproduction, embryo cyropreservation, and rental of a microinjection apparatus for investigators who want to perform their own microinjection experiments. This resource, which has been successful in meeting requests for its services, is expected to continue to provide both existing and new services as the demand for germline-altered mice in biomedical investigation continues to grow.