TUBERCULOSIS IN A MULTIETHNIC CITY POPULATION

该方案将确定艾滋病毒感染和未感染的注射吸毒者、注射吸毒者的性伴以及与感染结核病的成年人接触的儿童中结核分枝杆菌(M TB)感染的发生率。它将确定导致结核杆菌感染的环境和宿主风险因素；描述儿童和成人结核杆菌疾病的自然历史；调查特定结核杆菌菌株的流行病学；评估结核杆菌混合感染对艾滋病毒疾病进展速度的影响；以及调查影响结核病预防和治疗依从性的因素。该实验室用于血液分离、DNA分离、酶联免疫吸附试验、层流罩、寡核苷酸合成、聚合酶链式反应和RNA分离以及重组DNA技术。

This protocol will determine the incidence of Mycobacterium tuberculosis (M tb) infection in HIV-infected and -uninfected injecting drug users (IDUs), sexual partners of IDUs, and children in contact with TB-infected adults. It will identify environmental and host risk factors for developing M tb infection; describe the natural history of M tb disease in children and adults; investigate the epidemiology of specific strains of M tb; evaluate the effect of M tb coinfection on the rate of progression of HIV disease; and investigate factors influencing compliance with tuberculosis prophylaxis and therapy. The lab was used for blood separation, DNA isolation, ELISA, laminar flow hoods, Oligonucleotide synthesis, PCR, and RNA isolation, and recombinant DNA techniques.