DETECTION OF CANCEROUS CELLS VIA FT IR MICROSCOPY

描述(摘自摘要)：红外线的差异(振动) 正常细胞和癌细胞和组织之间的吸收光谱已经被 由几个研究小组报告。这项拟议的研究旨在利用 这些差异建立了一个基于光谱学的诊断工具 癌症的检测。红外光谱学和红外显微光谱学 承诺成为诊断这种疾病的理想工具，因为所有 细胞和组织中的生物分子表现出固有的和特定的红外 作为生化过程的探针或标记的光谱模式。 与其他诊断技术不同，红外光谱分析不需要 特定的外部探针来检测细胞成分的变化。在……里面 此外，可以区分成熟、分化、 细胞分裂周期的不同阶段和细胞的健康状态 光谱模式。对光谱结果的详细理解导致了 在更可靠、更客观、更基于仪器的设备中检测疾病的能力 接近。 正常和异常样本之间或类型之间的光谱差异 可以被描述为肉眼的光谱变化，这可能是由于 糖原耗竭对光谱贡献增加的结构 蛋白质或其他固有的标记分子。DNA/RNA的更微妙的变化 已观察到的光谱特征似乎是 癌症。 研究方案的总体目标是确立事业，发展 对DNA/RNA光谱区光谱差异的理解 正常样本和异常样本之间的差异。为了实现这一目标，一个 必须了解各种细胞和组织的光谱特征 进一步，以及光谱对细胞成熟状态的依赖 和分化，以及其他因素，如细胞凋亡、坏死和 细胞周期的阶段，需要建立。

DESCRIPTION (Adapted from abstract): Differences in the infrared (vibrational) absorption spectra between normal and cancerous cells and tissues have been reported by several research groups. The proposed research aims to utilize these differences to establish a spectroscopy-based diagnostic tool for the detection of cancer. Infrared spectroscopy and infrared microspectroscopy promise to be ideal tools for the diagnosis of this disease, since all biomolecules in cells and tissues exhibit inherent and specific infrared spectral patterns that act as probes, or markers, for biochemical processes. Infrared spectroscopy, unlike other diagnostic techniques, does not require specific external probes to detect changes in cellular composition. In addition, it is possible to distinguish states of maturation, differentiation, stages of the cell division cycle and state of health of cells by the changing spectral patterns. A detailed understanding of the spectral results leads to the ability to detect disease in a more reliable, objective, instrument-based approach. The spectral differences between normal and abnormal samples, or between types of tissue, can be described as gross spectral changes, which may be due to glycogen depletion of increase in the spectral contributions of structural proteins or other inherent marker molecules. More subtle changes in the DNA/RNA spectral signatures have been observed that appear to be specific signatures of cancer. The overall aim of the research proposal is to establish the cause, and develop the understanding of, the spectral differences in the DNA/RNA spectral regions between normal and abnormal samples. In order to achieve this goal, an understanding of the spectral features of various cells and tissues must be furthered, and the dependence of the spectra on the cell's state of maturation and differentiation, as well as other factors, such as apoptosis, necrosis and stage of the cell cycle, need to be established.