INVESTIGATION OF BETA HAIRPIN FORMATION IN WATER

我们感兴趣的是短肽的二级结构， 水溶液。 我们小组以前的研究表明， 一系列含有16聚体的D-Pro采用单体？发夹 构象在水溶液中，而它们的全-L立体异构体 没有 我们计划探索的基本要求？-发夹 在水中形成。 第一个目标是确定 最低数量的残留物所需的稳定？-发夹。 的 第二个目标是进行结构-活性研究， 研究特定的残基和残基间的相互作用 有助于b-发夹的稳定性。 设计为含有 还将分析多个发夹。 我们的团队以前 确认身份？在有机和水性介质中使用 NMR技术。 COSY和TOCSY实验促进峰值 分配，而NOESY和/或ROESY实验提供信息 关于三维解决方案结构。 额外 表征合成肽的实验技术 将包括圆二色性和IR研究。

We are interested in the secondary structure of short peptides in aqueous solutions. Previous studies within our group have shown that a series of D-Pro containing 16-mers adopt monomeric ?-hairpin conformations in aqueous solution, while their all-L stereoisomers do not. We plan to probe the fundamental requirements for ?-hairpin formation in water. The first objective will be to determine the minimum number of residues necessary for a stable ?-hairpin. The second goal will be to perform structure-activity studies with the aim of learning how specific residues and interresidue interactions contribute to b-hairpin stability. Peptides designed to contain multiple hairpins will also be analyzed. Our group has previously identified ?-hairpin formation in both organic and aqueous media using NMR techniques. COSY and TOCSY experiments facilitate peak assignments, while NOESY and/or ROESY experiments provide information regarding the three-dimensional solution structure. Additional experimental techniques for characterization of synthesized peptides will include circular dichroism and IR studies.