HYBRID LC/MS/MS QUADRUPOLE TOF MASS SPECTROMETER

俄勒冈州立大学化学系和环境健康科学中心（EHSC）向NCRR申请资金，购买一套新的Q-T型混合四极杆/正交飞行时间质谱仪和数据系统（MS/MS/DS）。该仪器需要填补俄勒冈州立大学现有能力的空白，以解决质谱实验室正在进行的问题。俄勒冈州立大学质谱实验室目前可用的仪器不具备解决我们日益遇到的许多蛋白质结构问题所需的灵敏度和分辨率。新仪器将支持的研究项目一般分为两个主要领域。这些研究包括：1)从二维凝胶点消化分析中鉴定蛋白质；2)对折叠中间体和由修饰和配体相互作用产生的构象状态进行氢/氘交换研究。此外，该仪器还将用于鉴定蛋白质修饰和磷酸化位点、肽序列和海洋生物碱结构。主要用户有NIH资助来开展应用程序中描述的研究。虽然EHSC、部门资金和设施费用为质谱分析设施提供了支持，但没有适当的机制来筹集足够的资金购买主要设备。新仪器的第二个作用是为那些需要高分辨率、质量精度和灵敏度的研究人员提供校园范围内的质谱支持。俄勒冈州立大学的质谱分析设施也支持俄勒冈州其他主要学术机构的质谱分析需求。

The Department of Chemistry and Environmental Health Sciences Center (EHSC) at Oregon State University requests funds from the NCRR to purchase a new Q-T of Hybrid Quadrupole/Orthogonal Time- of-Flight Mass Spectrometer and Data System (MS/MS/DS). This instrument is needed to fill a void in the existing capabilities at OSU to solve ongoing problems in the mass spectrometry laboratory. The instrumentation that is currently available in the OSU mass spectrometry laboratory does not have the combined sensitivity and resolving power necessary to solve many of the protein structural problems we are increasingly encountering. The research projects that will be supported by the new instrument fall generally into two major areas. These are 1) protein identification from analysis of 2-D gel spot digests and 2) hydrogen/deuterium exchange studies on folding intermediates and conformational states resulting from modifications and ligand interactions. In addition the instrument will be used for identifying sites of protein modifications and phosphorylation, peptide sequences, and marine alkaloid structures. The major users have NIH funding to carry out the studies described in the application. Although EHSC, departmental funds and facility charges have provided support for the mass spectrometry facility, there is no mechanism in place to raise sufficient funds to purchase major equipment. A secondary role of the new instrument will be to provide campus-wide mass spectrometry support for those investigators requiring high resolution, mass accuracy, and sensitivity. The OSU Mass Spectrometry Facility also supports the mass spectrometry needs of the other major Oregon academic institutions.