UV&IR MALDI REFLECTION TIME OF FLIGHT MASS SPECTROMETER

购买了布鲁克Apex III反射矩阵辅助激光解吸/电离（MALDI-TOF）质谱仪（MS），配备了紫外（337 nm）和红外（Er-YAG, 2.94 um）激光，脉冲离子提取和后源衰变（PSD），以满足波士顿大学（BU）医学院9个主要用户进行的6个项目的需求。波士顿大学戈德曼牙科医学院（都在波士顿大学医学中心校区）和马萨诸塞州沃尔瑟姆市尤尼斯肯尼迪施莱弗智力迟钝中心。这些美国国立卫生研究院资助的研究人员从事(1)确定导致心血管疾病的翻译后蛋白修饰的模板和性质（Cohen），(2)确定对新生儿和幼儿具有保护作用的牛奶低聚糖和糖蛋白的结构/活性关系（Newburg），(3)研究角膜中的蛋白聚糖（Nugent, Trinkaus-Randall），(4)鉴定存在于细胞膜中的蛋白质，肽和其他成分，牙齿表面的第一个攻击和防御部位（Oppenheim, Troxler），(5)了解暴露于香烟烟雾的蛋白质组学后果（Taccioli），以及(6)表征导致淀粉样蛋白疾病的沉积和循环蛋白，这些疾病是由骨髓惰性浆细胞病变（AL）后免疫球蛋白轻链片段的纤维形成引起的，血清淀粉样蛋白A（系统性淀粉样变性），在患有遗传性基因突变（家族性淀粉样变性多神经病变，FAP或ATTR）或高龄（老年性心脏淀粉样变性，SCA）的患者中（Walsh, Skinner）， BUMC和其他附近机构的六名次要用户也需要最先进的MALDI-TOF质谱分析，以支持阿尔茨海默病（Abraham），酶催化的过渡状态和中间体（Allen），蛋白质n -糖基化途径（Kukuruzinska），凝血酶受体（Kuliopoulos），向t细胞呈递非肽抗原（Moody）以及与动脉粥样硬化相关的脂质和载脂蛋白（Small）。Reflex III MALDI-TOF M将被安置在BUSM MS资源中。将优先考虑所述的项目；在剩余的时间里，北卡罗来纳大学和B主校区的未成年用户以及大型资源社区将得到服务。仪器将由实验室有经验的人员操作和维护。该资源在合作和教育方面有着良好的记录，并将确保该仪器系统的用户被纳入这些计划。

A Bruker Apex III reflectron matrix-assisted laser desorption/ionization (MALDI-TOF) mass spectrometer (MS) fitted with ultraviolet (nitrogen, 337 nm) and infrared (Er-YAG, 2.94 um) lasers, pulsed ion extraction and post-source decay (PSD) be purchased to serve the needs of six projects that are carried out by a group of nine major users based at Boston University (BU) School of Medicine, BU Goldman School of Dental Medicine (both at the BU Medical Center campus) and the Eunice Kennedy Shriver Center for Mental Retardation, Waltham, MA. These NIH-funded investigators are engaged in (1) determining the templates and nature of post-translational protein modifications leading to cardiovascular disease (Cohen), (2) determining the structure/activity relationships of milk oligosaccharides and glycoproteins that have protective activity for newborns and young children (Newburg), (3) studying proteoglycans in the cornea (Nugent, Trinkaus-Randall) (4) identifying proteins, peptides and other components present in pellicle, the first site of attack and defense on the tooth surface (Oppenheim, Troxler), (5) understanding the proteomic consequences of exposure to cigarette smoke (Taccioli), and (6) characterizing deposits and circulating proteins resulting in amyloid diseases that arise from fibrillogenesis of immunoglobin light chain fragments following indolent plasma cell dyscrasia in the bone marrow (AL), of serum amyloid A protein (systemic amyloidosis, AA) and of the serum protein transthyretin in patients having inherited genetic mutations (familial amyloidotic polyneuropathy, FAP or ATTR), or advanced age (senile cardiac amyloidosis, SCA) (Walsh, Skinner) A group of six minor users at BUMC and other nearby institutions also have need for state-of-the-art MALDI-TOF MS analysis to support studies in Alzheimer's Disease (Abraham), transition states and intermediates in enzyme catalysis (Allen), protein N-glycosylation pathways (Kukuruzinska), the thrombin receptor (Kuliopoulos), presentation of non-peptid antigens to T-cells (Moody) and lipids and apolipoproteins related to atherosclerosis (Small). The Reflex III MALDI-TOF M will be housed in the BUSM MS Resource. Priority will be given to the projects described; minor users at BUMC and the B main campus, as well as the large Resource community will be served in the remaining time. The instrument will be operated and maintained by experienced personnel already associated with the laboratory. The Resource has a strong record for collaboration and education and will assure that users of this instrument system are integrated into these programs.