FEASIBILITY OF KHZ FRAME RATE TWO PHOTON MICROSCOPY

一种测量绝对双光子吸收的方法 分子在溶液中的横截面()已经计算出来了。这个 脉冲红外激发产生的荧光与 由相同激发引起的荧光由一种 使用非线性晶体来获得(， 消除了对量子产额、收集知识的需要 效率，或专注力。我们已将此方法与 用我们设计的分子的相对方法进行测量 加州理工学院的Marder和Perry团队(请参阅 大的双光子截面)具有大的(值(5000 GM)。 我们对甲醇中罗丹明B的测量结果与Xu和Webb的测量结果吻合得很好 (1996年)。大的双光子截面)具有大的(值(5000 通用汽车)。我们对甲醇中罗丹明B的测量结果与徐和 Webb(1996)。克里斯·徐和瓦特·W·韦伯，《双光子测量》 分子荧光团的激发截面与690的数据 至1050 nm“，载于J.Opt.SoC.Am B 13(3)，481-491(1996)。

A method for measurement of the absolute two-photon absorption cross-section, (, of a molecule in solution has been developed. The ratio of fluorescence induced by pulsed infrared excitation to fluorescence induced by that same excitation doubled in frequency by a non-linear crystal is used to obtain an absolute value for (, eliminating the need for knowledge of the quantum yield, collection efficiency, or concentration. We have used this method in conjunction with our measurements by relative methods of molecules designed by the Marder and Perry groups at Caltech (see Measurement of Molecules with Large Two-Photon Cross-Sections) to have large ( values (5000 GM). Our measurements of Rhodamine B in MeOH agree well with Xu and Webb (1996). Large Two-Photon Cross-Sections) to have large ( values (5000 GM). Our measurements of Rhodamine B in MeOH agree well with Xu and Webb (1996). Chris Xu and Watt W. Webb, "Measurement of two-photon exitation cross-sections of molecular fluorophores with data from 690 to 1050 nm" in J. Opt. Soc. Am. B 13 (3), 481-491 (1996).