TIME RESOLVED FLUORESCENCE SPECT OF RED SHIFTED GREEN FLUORESCENT PROTEIN MUTANT

菌根真菌形成互惠共生关系 植物的根，在几乎所有陆地上都是最重要的 生态系统。然而，人们对这种细胞的细胞学知之甚少。 协会，更准确地说是真菌的细胞学。AMF产品 没有隔开的长菌丝(它们在细胞之间没有壁 菌丝)。AMF也是多核的(它们有2000-5000个核 每个孢子)，以及当AMF孢子萌发时(即产生新的 菌丝)集中在菌核中的核沿菌丝游动。它是 认为“漂移的”原子核对建立是必不可少的 共生(参与植物-真菌识别过程)， 然而，以前对它们的行为和生命周期一无所知 在与寄主植物会面后。已经开发出的技术 允许在组织培养中建立菌根植物。 菌根真菌菌丝生长过程中DAPI染色 以琼脂为基础的生长介质。使用多光子显微镜，我们已经 能够可视化植物根部与真菌接触的区域并研究 DAPI标记的细胞核在真菌菌丝中的运动

Mycorrhizal fungi form mutualistic symbiotic associations with plant roots and are of primary importance in nearly all terrestrial ecosystems. However, very little is known about the cytology of this association, more precisely the cytology of the fungus. AMF produce long hyphae which are not septate (they have no walls between cells in the hyphae). AMF are also multinucleate (they have 2000-5000 nuclei per spore) and when the AMF spores germinate (that is, produce new hyphae) the nuclei concentrated in them wander along the hyphe. It is thought that the "wandering" nuclei are essential to the establishment of the symbiosis (involved in plant-fungus recognition processes), however nothing is known about their behaviour and life cycle before and after meeting the host plant. Techniques have been developed that allow for the establishment of Mycorrhizal plants in tissue culture. The mycorrhizae fungal hyphae are stained with DAPI while growing in agar based growth medium. Using multi photon microscopy we have been able to visualize the plant root-fungi contact areas and study the movement of DAPI-labeled nuclei in fungal hyphae.