Core--Morphology

形态学核心被设计为组织病理学、免疫细胞化学、透射电子显微镜和项目教师图像分析要求的核心设施。核心设施包括进行项目1-4拟议研究所需的人员和设备。Core将进行灌注固定并取出脊髓进行光镜和电镜检查。核心人员将进行免疫细胞化学程序，以及开发新的程序，以显示额外的抗体。Core将处理用于塑料嵌入的组织，用于光镜和电子显微镜分析。高质量的薄片将由核心人员制作，并染色用于超微结构分析。已建立的图像分析程序将用于量化脊髓损伤区域和体积，以及选择性神经元损伤和白质病理。最后，这个核心将协助调查人员在暗室程序和生成的摄影幻灯片是印刷品。

The Morphology Core is designed to function as a Core facility for the histopathology, immunocytochemistry, transmission electron microscopy, and image analysis requirements for the Program Project faculty. The Core facilities consist of personnel and equipment needed to perform the proposed studies in Projects 1-4. The Core will perform perfusion fixation and removal of spinal cords for light and electron microscopic examination. Core personnel will carry out immunocytochemical procedures, as well as develop new procedures for the visualization of additional antibodies. The Core will process tissues for plastic embedding for both light and electron microscopic analysis. High quality thin sections will be produced by personnel within the Core and stained for ultrastructural analysis. Established image analysis procedures will be utilized to quantitate spinal cord injury areas and volumes, as well as selective neuronal damage and white matter pathology. Finally, this Core will assist investigators in darkroom procedures and the generation of photographic slides are prints.