EXERCISE AND FUEL METABOLISM

描述（改编自申请人摘要）：PI拟研究 运动中和运动后糖代谢的调节 和实验工具，使良好的控制实验进行 in vivo.实验将在长期插管的 吸收后狗在休息，跑步机运动和运动恢复期间， 手术和药理学技术来干扰或控制 糖调节系统葡萄糖通量和代谢的调节将是 使用同位素研究（葡萄糖、葡萄糖类似物和产气前体） 和动静脉差异（肝、肾和肠）技术。此外，本发明还提供了一种方法， 调节胰腺激素分泌和去甲肾上腺素溢出将是 使用胰腺动静脉差异测量。的具体目标 建议的实验是为了确定：（a）外周vs 门静脉高胰岛素血症抑制内源性葡萄糖生成 （B）在运动过程中监测所述组织的组成的解剖部位; 血液，允许在休息和运动期间精细控制血糖;（c） 运动是否会增强对选择性神经性低血糖症的反应，例如 它对低血糖的反应;和（d）通过先前的运动 增强肠道葡萄糖吸收和肝脏的内在能力 来消耗葡萄糖将拟议研究联系起来的共同线索是 目标是全面定义葡萄糖可用性的决定因素， 肌肉工作的代谢挑战增加。完成 拟议中的实验的目标将增加相当多的洞察力， 健康和糖尿病运动期间和之后的血糖调节知识。

DESCRIPTION (Adapted from the applicant's abstract): The PI proposes to study the regulation of glucose metabolism during and after exercise using surgical and experimental tools that allow well-controlled experiments to be carried out in vivo. Experiments will be conducted in chronically catheterized postabsorptive dogs during rest, treadmill exercise and exercise recovery using surgical and pharmacological techniques to perturb or control the glucoregulatory system. Regulation of glucose fluxes and metabolism will be studied using isotopic (glucose, glucose analogs and gluconeogenic precursors) and arteriovenous difference (liver, kidney and gut) techniques. In addition, regulation of pancreatic hormone secretion and norepinephrine spillover will be measured using pancreatic arteriovenous differences. The specific aims of the proposed experiments are to determine: (a) the significance of peripheral vs portal vein hyperinsulinemia in suppression of endogenous glucose production during exercise; (b) the anatomical site that monitors the composition of the blood, allowing for fine control of blood glucose during rest and exercise; (c) whether exercise heightens the response to selective neuroglycopenia, such as it does the response to hypoglycemia; and (d) the means by which prior exercise enhances intestinal glucose absorption and the intrinsic ability of the liver to consume glucose. The common thread that links the proposed studies is the goal of comprehensively defining determinants of glucose availability in the presence of the increased metabolic challenges of muscular work. Accomplishing the objectives of the proposed experiments will add considerable insight into knowledge of glucoregulation during and after exercise in health and diabetes.