BECTON-DICKINSON FACSCALIBUR FLOW CYTOMETRY SYSTEM

新的基因技术，加上具有良好特征的基因组，使小鼠成为人类疾病的首选哺乳动物模型。携带遗传预先确定的病理生物学综合征的小鼠株的产生和表征对生物医学研究至关重要，杰克逊实验室（TJL）的研究人员处于这一努力的前沿。鼠标模型的充分利用依赖于关键技术的获取。超过35%的TJL研究人员在免疫学、糖尿病、造血干细胞生物学、基因治疗和癌症研究领域高度依赖流式细胞术技术进行研究。流式细胞术是对单个细胞的膜和内部蛋白进行多参数定量测量、基因表达分析和细胞周期调控的重要工具。TJL目前的流式细胞仪设备负担过重，它变得太过时，无法满足我们NIH资助的研究项目的需求。基本的数据收集限制使得进行多参数分析的成本更高，从而限制了保持竞争力所必需的新兴流动技术的应用。Becton-Dickinson FACSCalibur流式细胞仪系统已在临床和研究环境中证明了其可靠性。FACSCalibur具有自动样品装入器、灵活高效的分选能力和用户友好的界面，是一种经济高效的解决方案，使研究人员能够最大限度地发挥其小鼠模型的潜力。申请资金用于购买FACSCalibur流式细胞仪系统，该系统将由该机构的流式细胞仪服务部门操作，从而提供一种具有成本效益的方式来满足TJL工作人员的紧急和未满足的需求。

New genetic technologies, combined with a well-characterized genome, make the mouse the preferred mammalian model for human disease. The generation and characterization of mouse strains harboring genetically predetermined pathobiological syndromes is critical to biomedical research, and researchers at the Jackson Laboratory (TJL) are at the forefront of this endeavor. Full exploitation of mouse models rely on access to critical technologies. Over 35% of TJL's research staff rely heavily on flow cytometric techniques to carry out studies in the areas of immunology, diabetes, hemopoietic stem cell biology, gene therapy and cancer research. Flow cytometry constitutes an essential tool for quantitative multiparameter measurements of membrane and internal proteins on individual cells, for gene expression analysis and cell cycle regulation. TJL's current flow cytometry instrumentation is overburdened and it is becoming too outmoded to meet the demands of our NIH funded research projects. The basic data collection limitations make it more costly to carryout multiparameter analyses and thus limit the application of emerging flow technologies necessary to remain competitive. The Becton-Dickinson FACSCalibur Flow Cytometry System has proven its reliability in both clinical and research settings. Because of its automated sample loader, flexible and efficient sorting capability and user friendly interface, the FACSCalibur is a cost-efficient solution enabling investigators to maximize the potential of their mouse models. Funds are requested to purchase the FACSCalibur Flow Cytometry System, to be operated by the institution's Flow Cytometry Service thus providing a cost-effective way to fill an urgent and unmet need of TJL Staff.