Core--Microarray facility

受体-配体相互作用和信号转导生物化学的教育是细胞生物学中所有事件的驱动力。在免疫系统中尤其如此，它具有许多受体-配体相互作用，促进细胞分化、增殖、凋亡和效应功能的激活。因此，信号转导生物化学是理解免疫细胞内稳态生物事件的核心。在过去的十年里，免疫细胞信号传导的知识体系已经相当成熟，特别是随着酪氨酸蛋白激酶的中心作用和由此产生的蛋白-蛋白相互作用的发现。由于这是一个新兴领域，用于研究细胞因子或其他免疫细胞激活剂诱导的信号事件的技术相当专业化，没有直接支持的经验不足的实验室可能不易获得。同样，考虑到一种受体与另一种受体的检测方法的相似性，人们希望从过去研究类似系统的人的经验中获益。因此，本申请提出了一个核心设施，以容纳专注于免疫细胞信号转导生物化学的专业知识和设备。它将被用作需要探索各种免疫反应中的信号事件的新研究人员的知识和技术资源，并将促进专门知识和特定分析方法的开发和转移到新的环境中。

Receptor-ligand interactions and the education of signal transduction biochemistry are the driving force for all events in cell biology. This is especially true in the immune system, which features numerous receptor- ligand interactions that promote cell-differentiation, proliferation, apoptosis, and activation of effector functions. Accordingly, signal transduction biochemistry is central to understanding the biological events within immune cell homeostasis. The body of knowledge of immune cell signaling has considerably matured over the past ten years, especially with the discovery of the central role for tyrosine protein kinases and the resulting protein-protein interactions. Since it is a nascent field, the techniques used to study signaling events induced by cytokines or other immune cell activators are fairly specialized and perhaps not readily acquired by inexperienced laboratories without direct support. Likewise, given the similarity of assays that apply from one receptor to another, one would hope to benefit from past experience of those studying a like system. Thus, this application proposes a core facility to house expertise and equipment that focuses on immune cell signal transduction biochemistry. It will be used as a knowledge-based and technical resource for new investigators who find a need to explore signaling events in various immune responses and will facilitate development and transfer of expertise and specific assays to a new setting.