MODELING OF LASER INDUCED FLUORESCENCE SPECTROSCOPY IN TISSUE

“主动力振型”的非线性建模方法 (Pdm)在核心项目#1中开发，应用于蜘蛛 机械感受器。根本的问题是对 支持机械转导的生物物理机制和 对这一过程的精确定量描述。这些实验 (由其他来源资助)使用宽带随机刺激并记录 胞内或胞外。调查结果： 产品数据管理方法在胞外和胞内的应用 录音显示了对结果的极好的预测能力 型号，远远超过了以前型号的能力。两个产品数据管理系统 已在这些初步研究中确定，对应于 两种生物物理机制：一种编码强度，另一种 编码率的变化。后一种产品数据管理允许机械感受器 在不同的工作点保持增益灵敏度(自适应)。 胞内和胞外检测结果的比较 数据也被制作出来了。这种通用的方法可以应用于 其他神经系统，因此，提供了一个强大的工具 生物科学领域的多样性和广度。

The nonlinear modeling methodology of "principal dynamic modes" (PDM) developed in Core Project #1, was applied to a spider mechanoreceptor. The fundamental question is the identification of the biophysical mechanisms that subserve mechanotransduction and the precise quantitative description of this process. The experiments (funded from other sources) employ broadband random stimuli and record either intracellularly or extracellularly. Results from the application of the PDM method to extracellular and intracellular recordings have shown excellent predictive ability of the resulting model, far exceeding the capabilities of previous models. Two PDM's have been identified in these preliminary studies, corresponding to two biophysical mechanisms: one that encodes intensity and another encoding rate of change. The latter PDM allows the mechanoreceptor to retain gain sensitivity at different operating points (adaptation). Comparisons between the results from intracellular and extracellular data were also made. This general approach can find application to other neural systems and, therefore, offers a powerful tool in a diverse and broad field of biological sciences.