DEVELOPMENT OF CELL POLARITY IN BUDDING YEAST

描述（来自申请人摘要的逐字记录）：S。酿酒厂是一个 由于其强大的生物学特性， 遗传学，它的简单性，观察极性发展的能力 在细胞群体中同步，以及存在许多突变和 细胞极性的分子标记研究将确定Cdc 42 GT3是如何 控制肌动蛋白组织，以及肌动蛋白如何介导极化内吞作用。 37个定点cdc 42突变体将在 透化的酵母和肌动蛋白组装能力的酵母提取物， 生物化学活性到体内功能，并用于遗传筛选， 识别下游途径的组成部分。Cdc 42效应器Pak的作用 激酶在形态发生中的作用将通过遗传和化学遗传学方法阐明， 战略布局在假菌丝生长中有缺陷的特殊cdc 42等位基因将是 研究，以提供更深入的了解真菌的分子基础， 二型性，这是许多真菌病原体的特征。最后，一个复杂的 一组相互作用的蛋白质将内吞膜连接到Arp 2/3复合物， 以及这些蛋白质是如何被本研究中发现的新型方舟激酶调节的。 实验室将进行调查。

DESCRIPTION (Verbatim from the applicant's abstract): S. cerevisiae is an excellent organism for studies of cell polarity development due to its powerful genetics, its simplicity, the ability to observe polarity development synchronously in a cell population, and existence of numerous mutations and molecular markers for cell polarity. Studies will determine how Cdc42 GTPase controls actin organization, and how actin mediates polarized endocytosis. Thirty-seven site-directed cdc42 mutants will be characterized biochemically in permeabilized yeast and in actin-assembly competent yeast extracts to relate biochemical activities to in vivo functions, and used in genetic screens to identify components of downstream pathways. Roles for Cdc42 effector Pak kinases in morphogenesis will be elucidated by genetic and chemical-genetic strategies. Special cdc42 alleles defective in pseudohyphal growth will be studied to provide a deeper understanding of the molecular basis for fungal dimorphism, a characteristic of many fungal pathogens. Finally, how a complex set of interacting proteins links endocytic membranes to the Arp2/3 complex, and how these proteins are regulated by novel Ark kinases discovered in this laboratory, will be investigated.