Workshop On Experimental Models from Bursa of Fabricius

描述（由申请人提供）：法布氏囊实验模型研讨会将于2002年10月4日至6日在华盛顿州西雅图市Fred Hutchinson癌症研究中心一家领先的生物医学研究机构的现代化会议设施举行。基于法氏囊及其衍生物的实验模型系统为研究具有广泛意义的生物学问题提供了高效的机会。例如：(1)癌基因如c-myc、c-myb和Rel诱导的肿瘤发病机制，包括血管生成和程序性细胞死亡的分析。(2) b细胞发育，包括免疫球蛋白基因多样化机制；(3)体细胞干细胞生物学和移植；(4)培养法氏囊源性（DT40）细胞的靶向基因缺失和替代。后一种技术，独特的用于培养脊椎动物细胞系，已被用于越来越多的应用范围内的新颖和揭示性实验，包括；lg基因多样化的重组和转化、b细胞受体的信号转导途径、RNA剪接的调节、着丝粒蛋白在染色体动力学中的功能以及进出DT40细胞的人类染色体的靶向诱变。尽管取得了这样的成就，但这些实验方法并没有像它们所代表的机会所期望的那样广为人知和应用。一个限制是缺乏鸡基因组资源来构建和分析法氏囊和DT40细胞的实验。因此，研讨会将重点介绍(5)目前可用和/或正在开发的用于这些系统的基因组资源的扩展阵列。研讨会将首次将使用这些相关实验系统的主要研究人员聚集在一起，以便彼此见面并分享他们的发现和专业知识。邀请了17位主要研究人员的核心演讲者。其余的演讲者和海报计划将从提交的摘要中选出，以回应一般公告。会议将向有兴趣的学生、博士后、实习生和科学家开放，名额不限。这次研讨会将有助于促进进一步的发展和应用，并教育有兴趣的年轻科学家了解所提供的机会。

DESCRIPTION (provided by applicant): The workshop on Experimental Models from the Bursa of Fabricius will be held October 4-6, 2002, in the modern conference facilities of a leading biomedical research institute, the Fred Hutchinson Cancer Research Center, in Seattle, Washington. Experimental model systems based on the bursa and its derivatives provide highly productive opportunities for study of biological problems of broad significance. Examples include: (1) pathogenesis of neoplasia induced by oncogenes such as c-myc, c-myb and Rel, including analysis of angiogenesis and programmed cell death. (2) B-cell development, including mechanisms of immunoglobulin gene diversification, (3) somatic stem cell biology and transplantation, (4) targeted gene deletion and replacement in cultured bursal-derived (DT40) cells. This latter technology, unique for use with cultured vertebrate cell lines, has been exploited for novel and revealing experiments in an increasing range of applications including; recombination and conversion in lg gene diversification, signal transduction pathways from B-cell receptors, regulation of RNA splicing, function of centromeric proteins in chromosome dynamics and targeted mutagenesis in human chromosomes shuttled in and out of DT40 cells. Despite such achievements these experimental approaches are not as widely known and applied as might be expected from the opportunity they represent. One limitation has been a lack of chicken genomic resources for both construction and analysis of experiments in the bursa and DT40 cells. Therefore, the workshop will feature (5) the expanded array of genomic resources now available and/or under development for use in these systems. The workshop will bring together, for the first time, leading investigators working with these related experimental systems in order to meet each other and share their findings and expertise. A core of 17 leading investigators are invited speakers. The remaining program of speakers and posters will be selected from submitted abstracts in response to a general announcement. The meeting will be open to interested students post-doctoral trainees and scientists up to the capacity of the facilities. This workshop will serve to stimulate further development and application, and to educate interested young scientists to the opportunities presented.