Core--Morphology

形态学核心旨在作为核心设施的组织病理学，免疫细胞化学，透射电子显微镜和图像分析的要求，为计划项目教师。核心设施包括开展项目1-4中拟议研究所需的人员和设备。Core将进行灌注固定和脊髓切除，以进行光学和电子显微镜检查。核心人员将进行免疫细胞化学程序，以及开发新的程序，为其他抗体的可视化。Core将处理用于塑料包埋的组织，以进行光学和电子显微镜分析。核心内的人员将制作高质量薄切片，并进行染色以进行超微结构分析。将利用已建立的图像分析程序对脊髓损伤面积和体积以及选择性神经元损伤和白色病变进行定量。最后，该核心将协助调查人员进行暗室程序，并生成照片幻灯片。

The Morphology Core is designed to function as a Core facility for the histopathology, immunocytochemistry, transmission electron microscopy, and image analysis requirements for the Program Project faculty. The Core facilities consist of personnel and equipment needed to perform the proposed studies in Projects 1-4. The Core will perform perfusion fixation and removal of spinal cords for light and electron microscopic examination. Core personnel will carry out immunocytochemical procedures, as well as develop new procedures for the visualization of additional antibodies. The Core will process tissues for plastic embedding for both light and electron microscopic analysis. High quality thin sections will be produced by personnel within the Core and stained for ultrastructural analysis. Established image analysis procedures will be utilized to quantitate spinal cord injury areas and volumes, as well as selective neuronal damage and white matter pathology. Finally, this Core will assist investigators in darkroom procedures and the generation of photographic slides are prints.