DEVELOPMENT OF A MOUSE MONITORING SYSTEM

描述（由申请人提供）：使用基因改变的小鼠， 成为一种标准的生物技术， 各种肽的生理作用和分子作用机制。 典型地，转基因或基因敲除小鼠的研究涉及以下的特定研究： 感兴趣的肽。然而，越来越明显的是， 通过转基因或敲除动物的肽表达的改变可 有意想不到的效果。在肥胖和II型糖尿病的情况下， 调节体重和葡萄糖稳态的分子的改变可能 导致整体能量稳态的变化，这可能是由于 进食、能量消耗的变化或激素敏感性的改变。这些 结果可能是预期的或不可预测的，因为靶向肽可能 与其他分子发生未知的相互作用。因此，在评估 转基因或敲除模型，除了检查 体重和胰岛素敏感性等参数，重要的是 通过量热法检查能量消耗和氧气消耗 以正确评估进食行为的模式。有九 参与肥胖症和糖尿病研究的研究人员积极地 使用了近50种不同的基因工程小鼠模型。许多 这些模型具有肥胖或瘦型表型，其必须涉及 进食或能量消耗或两者的变化。此应用程序用于 CLAMS，如果得到资助，将允许分析这些动物的能量消耗 和一般行为，以及允许精确监测喂养， 喝酒这种分析对于理解扰动是必不可少的， 导致肥胖和糖尿病。该工具的使用将与 由JDC国家卫生研究院（NIH）资助的动物核心 糖尿病和内分泌学研究中心（DERC）（DK 36836）， 越来越多地关注小鼠的生理和行为评价。

DESCRIPTION (Provided by applicant): The use of genetically altered mice has become a standard biological technique aimed at addressing both the physiologic role and molecular mechanism of action of various peptides. Typically studies of transgenic or knockout mice involve specific studies of the peptide of interest. However, it is increasingly apparent that alterations of peptide expression through transgenic or knockout animals may have unexpected effects. In the case of obesity and Type II diabetes, alterations in molecules regulating body weight and glucose homeostasis may lead to changes in overall energy homeostasis that may result from changes in feeding, changes in energy expenditure or altered hormone sensitivity. These results may be expected or unpredictable, as the targeted peptide may be involved in unknown interactions with other molecules. Hence, in evaluating transgenic or knockout models it is essential that in addition to examining parameters such as body weight and insulin sensitivity it is important to examine energy expenditure and oxygen consumption through calorimetry as well as to properly evaluate patterns of feeding behavior. There are nine investigators involved in research on obesity and diabetes who are actively using close to 50 different genetically engineered mouse models. Many of these models have an obese or lean phenotype, which must involve either changes in feeding or energy expenditure or both. This applications for the CLAMS, if funded, will allow analysis of energy expenditure in these animals and general behavior as well as allow precise monitoring of feeding and drinking. This analysis is essential for understanding the perturbations that lead to obesity and diabetes. Use of this instrument will be integrated with the Animal Core funded by the JDC National Institutes of Health (NIH)-funded Diabetes and Endocrinology Research Center (DERC) (DK 36836) which will be increasingly focused on physiologic and behavioral evaluation of mice.