Image-coded Microsphere for Multiplexed Bioassay

描述（由申请人提供）：由于基因组学和蛋白质组学的当前研究产生更多的多重数据，因此需要能够在非常小体积的样品中快速筛选大量核酸和蛋白质的技术。微阵列和DNA芯片是在平面平台上进行的，在相对大的芯片表面上分配小体积的液体样品经常遇到样品量不足或分布不均匀的问题。MSI建议开发一种微图像编码微球技术，用于使用前所未有的微体积样品进行数千次生物测定。该技术基于光学条形码颗粒，通过将纳米或微米图像插入乳胶珠壳中形成。这项技术的新奇在于，图像编码的珠子可以识别表面上发生的反应。每个微珠执行一个测试，因此作为一个单一的分析物分析仪。通过添加微粒的混合物，可以同时、简单、快速和廉价地测试数千种分析物。 第一阶段的工作将集中在图像编码粒子合成，显微图像记录，光学解码和DNA杂交分析。在第二阶段，该技术将应用于细胞分析，单核苷酸多态性（SNP）和基于DNA的癌症诊断。

DESCRIPTION (provided by applicant): As current research in genomics and proteomics produce more multiplexed data, there is a need for technologies that can rapidly screen a large number of nucleic acids and proteins in a very small volume of sample. Microarray and DNA chips are performed on a planar platform, distributing a small volume of liquid sample over a relatively large chip surface often encounter the problems of an insufficient sample amount or non-uniform distribution. MSI proposes to develop a micro-image-coded microsphere technology for multiplexed thousands of bioassays using an unprecedented level of micro-volume samples. The proposed technology is based on optically bar-coded particles, formed by inserting nano or micro images into a latex bead shell. The novelty of this technology is that image-coded beads identify which reaction is taking place on the surface. Each microbead performs one test, thus acting as a single analyte analyzer. By adding a mixture of micro particles, several thousands analytes can be tested simultaneously, easily, rapidly, and inexpensively. Phase I work will focus on image-coded particle synthesis, micro image recording, optical decoding, and DNA hybridization assays. In Phase II, the technology will be applied to cellular analysis, single nucleotide polymorphisms (SNP), and DNA-based cancer diagnostics.