Comparative Toxicogenomics Database

描述(由申请人提供)：在本R21/R33号申请中，我们建议 建立由五个NIEHS发起的比较毒理基因组学数据库 支持的海洋和淡水生物医学科学中心和其他 参与研究与人类相关的海洋序列的科学家 毒物学。我们的假设是，目前的快速扩张 高通量DNA测序的努力必须伴随着这一发展 将序列信息与生物和毒理学联系起来的数据库 数据以充分实现这些数据的价值并促进协作 在科学家中。该数据库的初步功能包括：(A) 与环境毒理学相关的基因和它们的 Genbank和Swiss Prot中的序列；(B)序列同源性断言 这些基因和基因来自其他海洋和淡水物种以及更高 脊椎动物；(C)从微阵列开发的毒理学数据。(D)支持 参考文献，包括已发表的文献和受限文献(非Medline) 毒理学数据(NIEHS毒理学中心机构简报报告 报告)，强调表达系统的功能研究以及与 环境毒理学和人类健康；(E)分子的可获得性 试剂(探针、克隆、引物、EST、抗体等)。具体目标 本项目的主要内容包括：(1)建立比较毒物基因组学数据库 使用从5个MFBS中心选择的毒理学感兴趣的DNA序列和 其他参与者；(2)为MFBS中心提供排序服务 R21阶段，以增加数据库中的序列数量；(3) 为参会人员组织毒理基因组学生物信息学会议 审查数据库开发和进展到R33阶段的建议。一个 这项提议的独特之处在于与贾南·埃皮格博士的密切合作， 比邻巴尔的杰克逊实验室生物信息学项目负责人 Harbor已经为老鼠建立了世界领先的生物信息学数据库 基因组计划。该项目支持DNA测序的快速发展 通过将序列数据链接到其他重要的生物和实验室来输出 信息和促进MFBS中心和其他机构之间的合作 参与其中的科学家。

DESCRIPTION (provided by applicant): In this R21/R33 application, we propose to establish a Comparative Toxicogenomics Database initiated by five NIEHS supported Marine and Freshwater Biomedical Sciences Centers and other participating scientists that focuses on marine sequences of relevance to human toxicology. Our hypothesis is that the present rapid expansion of high-throughput DNA sequencing efforts must be accompanied by the development of a database that links sequence information with biological and toxicological data to realize fully the value of these data and facilitate collaboration among scientists. Initial functions of this database will encompass: (A) Curated links between genes relevant to environmental toxicology and their sequences in Genbank and Swiss Prot; (B) Sequence homology assertions between these genes and genes from other marine and freshwater species and higher vertebrates; (C) Toxicologic data developed from microarrays. (D) Supporting references, including published literature as well as restricted (non-Medline) toxicology data (Institutional Bulletin Reports, NIEHS Toxicology Center Reports), emphasizing functional studies in expression systems and relevance to environmental toxicology and human health; (E) Availability of molecular reagents (probes, clones, primers, ESTs, Antibodies, etc,). The specific aims of this project include: (1) Establishing a comparative toxicogenomics database using selected DNA sequences of toxicological interest from 5 MFBS centers and other participants; (2) Providing sequencing services to MFBS Centers during the R21 phase to augment the number of sequences in the database; (3) Organizing a toxicogenomics bioinformatics conference for participants of this proposal to review database development and progression to the R33 phase. A unique feature of this proposal is the close collaboration with Dr Janan Eppig, Head of the Bioinformatics Program at The Jackson Laboratory in neighboring Bar Harbor who has established world-leading Biolnformatics databases for the Mouse Genome Project. This project supports the rapid advances in DNA sequencing output by linking sequence data to other important biological and laboratory information and promoting collaboration among MFBS Centers and other participating scientists.