SELENOPROTEIN P FUNCTION AND REGULATION OF EXPRESSION

硒是一种必需的微量金属， 抗氧化剂在营养中的作用这些研究的长远目标 是为了了解硒蛋白P在促进神经元存活中的作用， 保护神经细胞免受氧化应激。本申请提出 建立在关于新硒蛋白的最新发现的基础上， 硒蛋白P。提出了三个具体的目标：（1）研究硒蛋白P的功能， 硒蛋白P在神经元存活和抗氧化损伤中的作用 以及硒的吸收、储存和利用机制。这些 研究将使用神经元来源的细胞，用各种构建体转染 对硒蛋白P的功能结构域进行定位和鉴定 硒蛋白P的相互作用伙伴，提供保护的能力， 从氧化应激的角度，分析了硒的储存、吸收和积累机制 和利用。(2)硒蛋白P在重金属防护中的作用 毒性调查将研究重金属毒性的保护， 重金属对硒蛋白P表达的调控及共定位 重金属和硒的含量。(3)表达及 硒蛋白P在斑马鱼中的功能原位杂交和 斑马鱼硒蛋白P需要量的测定及其功能 将进行。

Selenium is an essential trace metal which has important antioxidant functions in nutrition. The long range objectives of these studies are to understand the role of selenoprotein P in promoting neuronal survival and protecting neuronal cells from oxidative stress. This application proposes to build upon recent discoveries with regard to the novel selenoprotein and selenoprotein P. Three specific aims are proposed: (1) To investigate the function of selenoprotein P in neuronal survival and protection from oxidative stress, and mechanism of selenium uptake, storage, and utilization. These studies will use cells of neuronal origin, transfected with various constructs of selenoproteins P, to do mapping of the functional domains and identification of interaction partners of selenoprotein P, the ability to provide protection from oxidative stress, and analysis for mechanism of selenium storage, uptake and utilization. (2) The role of selenoprotein P in protection from heavy metal toxicity. Investigations will study protection from heavy metal toxicity, regulation of selenoprotein P expression by heavy metals, and co-localization of heavy metals and selenium within transfected cells. (3) Expression and function of selenoprotein P in zebra fish. In situ hybridization and determination of requirements and function of selenoprotein P in Zebra fish will be conducted.