GENOME RESOURCES FOR MODEL AMPHIBIANS

描述(申请人提供)：火蜥蜴(尾两栖动物)是人类健康和疾病领域的重要模式生物 研究包括肢体再生、神经传递、视力、肾脏 功能、胚胎发生、心脏发育和嗅觉。的效用 如果基本基因组资源是 开发并提供给研究人员。建议的目标是 项目是表征和确定表达的基因组位置 来自再生肢体和视网膜的序列(EST)，两个局部火蜥蜴组织 在生物医学研究方面。此外，为了对…做出更广泛的贡献 蜥蜴研究人员，与之前发表的基因相对应 基因库中的火蜥蜴序列将被绘制出来。EST的鉴定 而基因组图的开发将是短期内的重要资源 术语，因为它将允许：(1)构建火蜥蜴EST数据库 具有特殊意义的性状；(2)待确定的基因同源性 火蜥蜴与人类之间的关系；(3)现有的 火蜥蜴突变体；(4)复合体的数量性状基因座分析 表型；(5)脊椎动物基因组进化的重建。使用 就更长远的目标而言，拟议的项目将促进 有助于识别火蜥蜴基因的额外资源 具有生物医学意义。

DESCRIPTION (Provided by applicant): Salamanders (urodele amphibians) are important model organisms in several areas of human health and disease research, including limb regeneration, neural transmission, vision, renal function, embryo genesis, heart development, and olfaction. The utility of salamanders would be greatly extended if fundamental genome resources were developed and made available to researchers. The objective of the proposed project is to characterize and determine the genomic position of expressed sequences(ESTs)from regenerating limb and retina, two focal salamander tissues in biomedical research. Also, to make a more general contribution to salamander researchers, genes that correspond to previously published salamander sequences in Genbank will be mapped. The identification of ESTs and development of a genome map will be an important resource over the short term because it will allow: (1) construction of an EST database for salamander traits that are of special interest; (2) gene homologies to be determined between salamander and human; (3) candidate gene studies of existing salamander mutants; (4) quantitative trait locus analyses of complex phenotypes; and (5) reconstruction of vertebrate genome evolution. With respect to longer term goals, the proposed project will spur development of additional resources that will facilitate identification of salamander genes of biomedical significance.