Core--analytical biomarkers (xenobiotics) facility

描述（由申请人提供） 分析核心将为这三个项目提供研究支持。 中心支持的研究项目。它提供了一个多样化的阵列 分析仪器和专门知识，可用于 所有参与这个项目的科学家核心还提供专业知识， 为广泛的客户提供一次性或例行的分析支持 从亲脂性异生剂到生物聚合物的各种分子。 可用的技术包括各种色谱系统， 质谱仪和免疫化学。编码 从项目I采集的全血样本和其他组织样本 将被转移到核心I，如申请中详细描述的。 根据要进行的分析类型，全血 (e.g.,重金属分析）或血清将被提取以执行 测试如下所示。具体而言，研究人员将提供 分析支持，以测试关于已知 异生物质免疫毒素和神经毒素与自闭症。特别 注意力将集中在详细分析多卤代 有机（例如，多氯联苯、多溴联苯和聚偏二苯乙烷）、金属分析（例如，汞， 铅、铜、锌等）以及被怀疑 在加州有神经毒性和环境问题。的 研究人员还将提供高通量的食品分析， 血清和组织脂质来检验自闭症患者 条件可能与饮食脂质或血清脂质 简档可以指示自闭症状态。该实验室将 在提供高通量色谱方面具有支持功能， 物理和免疫化学检测的研究项目。它将 在改进分析技术方面具有研究支持功能 开发中心要求的新分析技术 investigators.最后，它将具有培训功能， 将分析核心的能力传达给项目 科学家和培训需要动手使用的项目科学家 用于项目研究的分析核心仪器。

DESCRIPTION (provided by applicant) The Analytical Core will provide research support for each of the three research projects supported by the Center. It provides a diverse array of analytical instruments and expertise that are available for use to all scientists on the project. The core also provides the expertise to offer analytical support on a one time or routine basis for a wide variety of molecules from lipophilic xenobitics to biological polymers. The technologies available include a variety of chromatographic systems, spectrophotometers, mass spectrometry, and immunochemistry. Coded samples of whole blood and other tissue samples collected from Project I will be transferred to Core I as described in detail in the application. Depending on the type of analysis to be performed, either whole blood (e.g., heavy metal profiling) or serum will be extracted to perform tests indicated below. Specifically the investigators will provide the analytical support to test hypotheses regarding the association of known xenobiotic immunotoxicants and neurotoxicants with autism. Particular attention will be focused at detailed analysis of polyhalogenated organic (e.g., PCBs, PBBS, and PDBEs), metals profiling (e.g., mercury, lead, copper, zinc, etc.) and priority pesticides suspected of being neurotoxic and of environmental concern in California. The investigators will also provide a high throughput analysis of food, serum, and tissue lipids to test the hypothesis that the autistic condition may be associated with dietary lipids or that the serum lipid profile may be indicative of the autistic state. The laboratory will have a support function in providing high throughput chromatographic, physical, and immunochemical assays for the research projects. It will have a research support function in improving analytical technologies and developing new analytical technologies requested by center investigators. Finally, it will have a training function in communicating the capabilities of the Analytical Core to project scientists and training scientists from projects who need hands-on use of the Analytical Core's instruments for project research.