GENOME RESOURCES FOR MODEL AMPHIBIANS

描述（由申请人提供）：蝾螈（有尾目两栖动物）是 人类健康和疾病若干领域的重要模式生物 研究，包括肢体再生，神经传输，视觉，肾脏 功能、胚胎发生、心脏发育和嗅觉。 的效用 如果基本的基因组资源能够被 开发并提供给研究人员。 建议的目标 项目是表征和确定表达的基因组位置， 再生肢体和视网膜，两个焦点蝾螈组织的EST序列 在生物医学研究中。 此外，为了更全面地促进 蝾螈研究人员，基因对应于先前发表的 将绘制基因库中的蝾螈序列。 EST的识别 基因组图谱的开发将是短期内的重要资源 这是因为它将允许：（1）建立蝾螈EST数据库 特别感兴趣的性状;（2）待确定的基因同源性 蝾螈与人类之间的差异;（3）现有的候选基因研究 蝾螈突变体;（4）复杂的数量性状基因座分析 表型;（5）脊椎动物基因组进化的重建。 与 就长远目标而言，拟议项目将促进 有助于识别蝾螈基因的额外资源 具有生物医学意义。

DESCRIPTION (Provided by applicant): Salamanders (urodele amphibians) are important model organisms in several areas of human health and disease research, including limb regeneration, neural transmission, vision, renal function, embryo genesis, heart development, and olfaction. The utility of salamanders would be greatly extended if fundamental genome resources were developed and made available to researchers. The objective of the proposed project is to characterize and determine the genomic position of expressed sequences(ESTs)from regenerating limb and retina, two focal salamander tissues in biomedical research. Also, to make a more general contribution to salamander researchers, genes that correspond to previously published salamander sequences in Genbank will be mapped. The identification of ESTs and development of a genome map will be an important resource over the short term because it will allow: (1) construction of an EST database for salamander traits that are of special interest; (2) gene homologies to be determined between salamander and human; (3) candidate gene studies of existing salamander mutants; (4) quantitative trait locus analyses of complex phenotypes; and (5) reconstruction of vertebrate genome evolution. With respect to longer term goals, the proposed project will spur development of additional resources that will facilitate identification of salamander genes of biomedical significance.