Diabetes, Exercise and Postprandial Oxidative Stress

描述（申请人提供）：餐后氧化应激和碳水化合物（CHO）和脂质代谢紊乱都与2型糖尿病并发症的发生有关。然而，有氧运动改善这些紊乱的能力尚未得到充分探讨。本研究将检测急性有氧运动（以65% VO2Max在跑步机上行走30分钟）对一组年轻发病的2型糖尿病和非糖尿病对照组的影响：(1)3餐后CHO和脂质代谢（葡萄糖、胰岛素、c肽和甘油三酯）；(2)三餐后的餐后氧化应激（总丙二醛、F2异前列腺素、总氢过氧化物、维生素A、E和类胡萝卜素）和LDL对氧化的敏感性（滞后时间，最大共轭二烯形成）；(3)急性有氧运动30分钟后的氧化应激和LDL对氧化的易感性。此外，还将探讨基线胰岛素敏感性、抗氧化状态、VO2Max与运动诱导的氧化应激、CHO和脂质代谢变化之间的关系。该研究将包括35名2型糖尿病患者（年龄18-40岁），糖尿病病程（<7年）和35名非糖尿病对照者，他们将在普通临床研究中心按随机顺序参加非运动（NE）和运动(E)方案。每个协议将持续三天。在任一方案的第一天，葡萄糖钳将用于测量胰岛素敏感性。在E方案的第二天，将在急性有氧运动之前和在运动后期间周期性地获得氧化应激、LDL对氧化的易感性和脂质代谢的基线参数，在此期间将消耗三餐。在NE方案的第二天，将遵循相同的方案，不进行急性运动回合。在每个方案的第3天上午（E期或NE期后24小时），绘制CHO、脂质和氧化应激的空腹参数。饮食将遵循国家胆固醇教育计划指南，30%的脂肪作为能量，10:10:10%来自饱和脂肪，单不饱和脂肪和多不饱和脂肪，每餐分别消耗三分之一的能量需求。本研究代表了一种利用有氧运动治疗2型糖尿病的新方法，为该领域未来的调查和运动训练研究提供了数据。

DESCRIPTION (provided by applicant): Both postprandial oxidative stress and derangements in carbohydrate (CHO) and lipid metabolism have been implicated in the development of complications in type 2 DM. Yet the ability of aerobic exercise to improve these derangements has not been fully explored. This study will examine the effects of an acute bout of aerobic exercise (30 minutes of treadmill walking at 65% VO2Max) in a group of physically untrained subjects with younger-onset type 2 DM and non-DM controls on: (1) CHO and lipid metabolism (glucose, insulin, C-peptides and triglycerides) following 3 meals; (2) postprandial oxidative stress (total MDA, F2 Isoprostanes, total hydroperoxides, vitamin A, E and carotenoids) and LDL susceptibility to oxidation (lag time, maximal conjugated diene formation) following 3 meals; and (3) oxidative stress and LDL susceptibility to oxidation 30 minutes after an acute bout of aerobic exercise. In addition, the relationship between baseline insulin sensitivity, antioxidant status, VO2Max and exercise-induced changes in oxidative stress, CHO and lipid metabolism will be explored. The study will include 35 subjects with type 2 DM (ages 18-40) with duration of diabetes (<7 years) and 35 non-DM controls who will participate in a nonexercise (NE) and an exercise (E) protocols, performed in random order at the General Clinical Research Center. Each of the protocols will be three days in duration. On the first day of either protocol, a glucose clamp will be performed for measurement of insulin sensitivity. On the second day of the E protocol, baseline parameters of oxidative stress, LDL susceptibility to oxidation and lipid metabolism will be obtained prior to an acute bout of aerobic exercise and periodically throughout the post exercise period, during which three meals will be consumed. On the second day of the NE protocol the same protocol will be followed without the acute exercise bout. On the morning of the third day of each protocol (24 hours after the E or NE session), fasting parameters of CHO, lipid and oxidative stress will be drawn. The diet will follow the National Cholesterol Education Program guidelines with 30% fat as energy, with 10:10:10% from saturated, monounsaturated and polyunsaturated fat, respectively with 1/3 of energy requirements consumed at each meal. This study represent a novel approach to the use of aerobic exercise in the treatment of type 2 DM, providing data for future investigations and exercise training studies in this area.