Viral Vector Core

血液研究所的病媒核心实验室将提供完成本申请中所述项目的具体目标所需的病毒病媒。这项拟议的研究需要在细胞培养和小鼠模型系统中高效地传递和表达流感病毒基因。载体核心将把单个流感病毒基因、亚基因组片段和突变基因克隆到牛痘病毒和基于腺病毒的病毒载体系统的穿梭载体中。核心实验室将使用标准方法将穿梭载体中介重组到腺病毒或牛痘病毒的基因组中，以创建重组病毒载体。核心实验室人员将与调查人员合作，选择最适合他们需求的重组病毒分离株。然后，核心实验室将放大和纯化病毒库存，并为要求构建载体的实验室提供病毒滴度。因此，媒介核心实验室将最大限度地满足研究人员的需求，为他们的研究计划提供一个强大而必要的工具，而不需要他们成为创建病毒载体的专家。

The vector core laboratory at the Blood Research Institute will provide the viral vectors required to complete the specific aims of the projects described in this application. The proposed research requires efficient delivery and expression of influenza virus genes in both cell culture and mouse model systems. The vector core will clone individual influenza virus genes, subgenomic fragments and mutated genes into shuttle vectors for both vaccinia virus and adenovirus-based viral vector systems. The core lab will use standard methods to mediate recombination of the shuttle vectors into the genome of either adenovirus or vaccinia virus to create a recombinant virus vector. The Core Lab personnel will work with the investigators to select the recombinant virus isolates that best suits their needs. The core lab will then amplify and purify the virus stock and provide a virus titer for the labs requesting the vector be constructed. The vector core lab will therefore function to best serve the needs of investigators by providing them with a powerful and necessary tool for their research programs without the need for them to become experts in the creation of viral vectors.