Intra-Oral Biofilm Formation

描述（由申请人提供）：影响口腔的主要问题是由生活在硬组织和软组织生物膜中的微生物引起的。虽然已经了解了很多关于成熟的牙菌斑，很少有人知道的顺序发生在龈上或龈下菌斑的发展过程中的微生物物种的变化。对软组织微生物群以及生物膜发育期间软组织微生物群和硬组织微生物群之间的相互作用的了解更少。两个重要的散装流体，唾液和GCF，洗澡的生物膜表面，并发挥生物膜的发展和未确定的作用。因此，本申请的目的是定义在早期生物膜再发育期间发生的微生物物种的变化，并评估可能影响这种发育的因素，包括唾液流量减少、缺乏天然牙列和牙龈炎症状态的变化。 SAl将比较60名牙周健康、60名牙周炎和60名戴假牙的无牙受试者从最初的牙齿清洁到7天在硬组织和软组织上形成生物膜的微生物组成的变化。将分别从基线时每颗牙齿的近中面采集龈上和龈下菌斑样本，并从假牙上采集龈上样本。还将采集唾液和8个口腔软组织表面的样本。将使用棋盘DNA-DNA杂交法单独评价样品中40种细菌的含量。在初始监测后，每组中的所有有牙受试者将接受牙齿清洁，而假牙将被清洁和消毒。每组随机抽取30名受试者，进行全口软组织清洁消毒。受试者将在7天内避免口腔卫生，在此期间，将在第1、2、4和7天从随机选择的象限采集龈上和龈下菌斑样本。在与唾液和软组织样本相同的点采集牙菌斑样本。SA 2将比较60名干燥综合征受试者和60名匹配对照者的龈上和龈下牙菌斑、口腔软组织和唾液的微生物组成。将评估全口龋齿和牙周状况，并从基线时每颗牙齿的近中面采集龈上和龈下菌斑样本。此外，还将采集8份软组织样本和1份唾液样本。所有样品将按照上述方法进行微生物评价。所有受试者将接受全口SAP，并将在第28天重新进行微生物学评估。拟议的研究应表明在硬组织和软组织上生物膜发育过程中发生的微生物变化的性质，以及唾液、GCF和天然牙列对发育中的生物膜的影响。

DESCRIPTION (provided by applicant): The major problems that affect the oral cavity are caused by organisms that live in biofilms colonizing the hard and soft tissues. While much has been learned about mature Dental plaque, little is known about the sequence of changes in microbial species that occurs during supra or subgingival plaque development. Even less is know about the soft tissue microbiota and the interplay between soft tissue microbiota and hard tissue microbiota during biofilm development. Two important bulk fluids, saliva and GCF, bathe the biofilm surfaces and play and undetermined role in biofilm development. Thus, the objectives of this application are to define the changes in microbial species that occur during early biofilm re-development and to evaluate factors that might influence this development including diminished salivary flow, lack of natural dentition and changes in gingival inflammatory status. SAl will compare the changes in microbial composition of developing biofilms on hard and soft tissues in 60 periodontally healthy, 60 periodontitis, and 60 denture-wearing, edentulous subjects from initial Dental cleaning to 7 days. Supra and subgingival plaque samples will be taken separately from the mesial aspect of each tooth at baseline and supragingival samples from dentures. Saliva and samples from eight oral soft tissue surfaces will also be collected. Samples will be evaluated individually for their content of 40 bacterial species using checkerboard DNA-DNA hybridization. After initial monitoring, all dentate subjects in each group will receive a Dental cleaning while dentures will be cleaned and disinfected. Thirty randomly selected subjects in each group will receive full mouth "soft tissue cleaning and disinfection. Subjects will refrain from oral hygiene for 7 days during which time Supra and subgingival plaque samples will be taken from randomly selected quadrants at 1, 2, 4, and 7 days. Denture plaque samples will be taken at the same points as saliva and soft tissue samples.SA2 will compare the microbial composition of supra and subgingival plaque, oral soft tissues, and saliva in 60 Sj¿gren's subjects and 60 matched controls. Full-mouth caries and periodontal status will be assessed and samples of supra and subgingival plaque will be taken from the mesial aspect of each tooth at baseline. In addition, eight soft tissue samples and a saliva sample will be taken. All samples will be evaluated microbiologically as described above. All subjects will receive full mouth SAP and will be re-assessed microbiologically at 28 days. The proposed study should indicate the nature of the microbial changes that occur during biofilm development on hard and soft tissues and the influence that saliva, GCF and the natural dentition have on the developing biofilms.