BD FACSARIA FLOW CYTOMETER 3 LSR

描述(由申请人提供)：此申请是为购买FACSAria细胞分选器而提出的资金申请。该仪器将被安置在匹兹堡大学新成立的免疫学系内。目前还没有细胞分选机可供系内的初级和中级教员使用，因此这项购买将对匹兹堡大学的免疫学社区产生巨大的影响。匹兹堡大学现有的细胞分选器要么不对系成员开放，要么距离系几英里远。进行高速细胞分选的能力是许多免疫学研究的一个基本特征。因此，这一应用的原因是：1)提供急需的工具；2)为初级和次级免疫学教师提供易于使用的细胞分类器；以及3)形成新创建的免疫学FLOW设施的基础。2002年1月成立了一个新的免疫学系，以表彰匹兹堡大学在这里进行的免疫学研究的实力。目前，免疫学系有30名初级和中级教员，其中大部分是由NIH资助的。购置所要求的细胞分选机将使个人用户能够获得细胞分选，而目前新闻部的许多成员还不能进行细胞分选。

DESCRIPTION (provided by applicant): This request is for funds to purchase a FACSAria cell sorter. This instrument will be housed within the newly created Department of Immunology at the University of Pittsburgh. At present there is no cell sorter available for use by primary and many of the secondary faculty in the Department and thus this purchase will have an enormous impact on the Immunology community at the University of Pittsburgh. Existing cell sorters at the University of Pittsburgh are either not available to Department members or are located several miles away from the Department. The ability to perform high speed cell sorting is an essential feature of much immunology research. Thus the reasons for this application are to: 1) Provide a critically needed instrument; 2) provide an easy-to-use readily accessible cell sorter for primary and secondary Immunology faculty; and 3) form the basis of the newly created Immunology Flow Facility. In January 2002 a new Department of Immunology was created in recognition of the strength of the immunology research performed here at the University of Pittsburgh. At present there are 30 primary and secondary faculty in the Department of Immunology and most of them are NIH-funded. The acquisition of the requested cell sorter will allow individual users access to cell sorting, which is at present unavailable to many members of the Department.