FACS Vantage SE

描述（由申请人提供）：本申请旨在请求支持为明尼苏达大学新成立的干细胞研究所的流式细胞术实验室购买具有FACSDiVa、Turbosort和Clonsort选项的FACSVantage SE。明尼苏达大学的干细胞研究所成立于1999年，其目标是（A）在遗传学和基因组学、发育生物学、干细胞及其分化后代的细胞生物学和生理学（B）将基础研究转化为遗传和变性疾病的新疗法（C）应用新的干细胞-通过美国已经建立的临床项目，明尼苏达州。在未来3年内，干细胞研究所将招募多达13名新教师，其中4人已被招募。需要一台新的仪器，因为两台现有仪器中的一台，即目前主要由干细胞生物学领域研究人员使用的FACStar plus，不再适合通过NIH资助的正在进行的研究（例如罕见事件分选，5-8色分选，4向分选），并且重建的原型可能不再可用。收购FACSDiVa选项、TurboSort选项和Clonsort Plus选项的FACSVantage Se将大大提高明尼苏达大学现有研究人员执行NIH资助研究项目的能力。它还将提供已经在明尼苏达大学开始的研究人员，并将在未来2-3年内招募，以进行技术复杂的高要求和准确的基于流式细胞术的研究，以进一步提高我们在干细胞生物学和相关领域的知识。

DESCRIPTION (provided by applicant): This application is to request support for the acquisition of a FACSVantage SE with FACSDiVa, Turbosort and Clonecyt options for the Flow Cytometry Laboratory in the newly established Stem Cell Institute at the University of Minnesota. The Stem Cell Institute at the University of Minnesota, which was created in 1999 has as objectives to (A) build further basic research in genetics and genomics, developmental biology, cell biology and physiology of stem cells and their differentiated progeny (B) translate basic research into novel therapies for genetic and degenerative disorders (C) apply new stem cell-based therapies in the clinic through already established clinical programs at the U. of Minnesota. A total of up to 13 new faculty will be recruited to the Stem Cell Institute over the next 3 years, 4 of whom have been recruited. A new instrument is requested because one of the two existing instruments, a FACStar plus, which is currently being used chiefly by investigators working in the area of stem cell biology is no longer adequate for ongoing studies that are funded through the NIH (for instance rare event sorting, 5-8 color sorting, 4-way sorting) and the prototype, that was rebuilt may no longer be serviceable. Acquisition of the FACSVantage Se with FACSDiVa option, TurboSort option and Clonecyt Plus option will greatly enhance the capability of established investigators at the University of Minnesota to perform NIH funded research projects. It will also provide beginning investigators already at the University of Minnesota and to be recruited over the next 2-3 years to perform technically sophisticated demanding, and accurate flow cytometry-based studies to further enhance our knowledge in stem cell biology, and related fields.