ANALYSIS OF ROLE OF CHD PROTEINS IN ARABIDOPSIS THALIANA

描述(来自应用程序的逐字)：此应用程序的目标 了解CHD蛋白在决定发育过程中的作用 拟南芥中的身份。CHD蛋白被认为与 染色质重塑因子存在于许多不同的生物体中，从 酵母传给人类。在拟南芥中，PKL突变存在缺陷 赤霉素信号转导途径及其在发育转型中的作用 从胚胎到幼苗。PKL已被克隆并编码为CHD家族成员。 我们有兴趣了解这种CHD蛋白在调控中的作用。 发育同一性和GA信号转导。以确定可能的 PKL调控机制、PKL转录本和蛋白的表达 将被描述为。基因将被识别为直接受 PKL，因此可能参与发展同一性的调节 和GA信号转导。与PKL相互作用的蛋白质将被鉴定 通过双杂交分析和亲和层析试图鉴定 可能调节PKL活性的潜在辅助因素。其他因素 参与发育同一性的调节，赤霉素信号 转导和/或染色质重塑可以基于基因 屏幕上。通过实现这些实验目标，希望有一个更大的 我们将了解CHD蛋白在调节 植物的发育，也许还有所有真核生物的发育。

DESCRIPTION (Verbatim from the application): The objective of this application is to understand the role of a CHD protein in determination of developmental identity in Arabidopsis thaliana. CHD proteins have been implicated as chromatin remodeling factors are found in many diverse organisms ranging from yeast to man. In Arabidopsis thaliana, the pkl mutation is defective in the gibberellin signal transduction pathway and in the developmental transition form embryo to seedling. PKL has been cloned and codes for a CHD family member. We are interested in understanding the role of this CHD protein in regulation of developmental identity and GA signal transduction. To identify possible mechanisms by which PKL is regulated, expression of PKL transcript and protein will be characterized. Genes will be identified that are directly regulated by PKL and therefore potentially involved in regulation of developmental identity and GA signal transduction. Proteins that interact with PKL will be identified by two-hybrid analysis and affinity chromatography in an attempt to identify potential co-factors that may regulate PKL activity. Additional factors that are involved in regulation of developmental identity, gibberellin signal transduction, and/or chromatin remodeling may be identified based on a genetic screen. By carrying out these experimental aims, it is hoped that a greater understanding will be achieved of the role of CHD proteins in regulation of development in plants and perhaps all eukaryotes.