Microcoil-based Probe for Protein NMR

描述(申请人提供)：这项工作的目标是创造一种新的基于微线圈的核磁共振检测探针，专门针对蛋白质分析。提交组织将利用一种获得专利和独家许可的方法进行敏感性匹配，该方法能够使用基于微线圈的探头进行小分子分析。微线圈对蛋白质分析的基本贡献是能够使用更少的样品、微克而不是毫克来获取数据。这为蛋白质化学家提供了一种核磁共振测量解决方案，该解决方案通常通过需要较少表达的蛋白质来节省时间，并适应具有潜在高价值但潜在较低产量的表达技术。根据这项SBIR开发的探针将设计成与(质子)频率范围从400 MHz-750 MHz的标准核磁共振光谱仪对接，并与快速增长的蛋白质组学领域现有的和正在开发的样品管理和分析分离技术相连接。紧随其后的市场将是制药和药物化学行业。

DESCRIPTION (provided by applicant): The goal of this effort is the creation of a new line of microcoil-based NMR detection probes specifically targeted to protein analysis. The submitting organization will utilize a patented and exclusively licensed approach to susceptibility matching that has enabled small molecule analysis using microcoil-based probes. The fundamental contribution of microcoils to protein analysis is the ability to acquire data using less sample, micrograms rather than milligrams. This provides the protein chemist with an NMR measurement solution that generally saves time by requiring less expressed protein, and that accommodates expression techniques that are of potentially high value but potentially lower yield. The probes developed under this SBIR will be designed to interface to standard NMR spectrometers ranging in (proton) frequency from 400 MHz - 750 MHz, and to sample management and analytical separations technologies both in existence and under development in the rapidly growing field of proteomics. The immediate market will be the pharmaceutical and medicinal chemistry industries.