Diabetes, Exercise and Postprandial Oxidative Stress

描述（由申请人提供）：餐后氧化应激和碳水化合物（CHO）和脂质代谢紊乱均与2型DM并发症的发生有关。 然而，有氧运动改善这些紊乱的能力还没有得到充分的探讨。 本研究将探讨急性一轮有氧运动的影响（在65%VO2Max条件下进行30分钟的踏车行走），研究对象为一组未接受过身体训练的2型糖尿病患者和非糖尿病对照组：（1）CHO和脂质代谢（葡萄糖、胰岛素、C肽和甘油三酯）;（2）餐后氧化应激（总MDA、F2异前列烷、总氢过氧化物、维生素A、E和类胡萝卜素）和LDL对氧化的敏感性（滞后时间，最大共轭二烯形成）;和（3）急性有氧运动后30分钟的氧化应激和LDL对氧化的敏感性。 此外，还将探讨基线胰岛素敏感性、抗氧化状态、最大摄氧量与运动诱导的氧化应激、CHO和脂质代谢变化之间的关系。 该研究将包括35名患有2型糖尿病（18-40岁）且糖尿病持续时间（<7年）的受试者和35名非糖尿病对照者，他们将参加在综合临床研究中心以随机顺序进行的非运动（NE）和运动（E）方案。 每项议定书为期三天。 在任一方案的第一天，将进行葡萄糖钳夹试验以测量胰岛素敏感性。 在E方案的第二天，将在急性有氧运动之前和运动后期间定期获得氧化应激、LDL对氧化的敏感性和脂质代谢的基线参数，在运动后期间将消耗三餐。 在NE方案的第二天，将遵循相同的方案，不进行急性运动回合。 在每个方案的第三天早晨（E或NE治疗后24小时），将采集CHO、脂质和氧化应激的空腹参数。 饮食将遵循国家胆固醇教育计划指南，30%的脂肪作为能量，10：10：10%来自饱和脂肪，单不饱和脂肪和多不饱和脂肪，每餐消耗1/3的能量需求。 本研究为有氧运动治疗2型糖尿病提供了一种新的方法，为今后在这一领域的调查和运动训练研究提供了数据。

DESCRIPTION (provided by applicant): Both postprandial oxidative stress and derangements in carbohydrate (CHO) and lipid metabolism have been implicated in the development of complications in type 2 DM. Yet the ability of aerobic exercise to improve these derangements has not been fully explored. This study will examine the effects of an acute bout of aerobic exercise (30 minutes of treadmill walking at 65% VO2Max) in a group of physically untrained subjects with younger-onset type 2 DM and non-DM controls on: (1) CHO and lipid metabolism (glucose, insulin, C-peptides and triglycerides) following 3 meals; (2) postprandial oxidative stress (total MDA, F2 Isoprostanes, total hydroperoxides, vitamin A, E and carotenoids) and LDL susceptibility to oxidation (lag time, maximal conjugated diene formation) following 3 meals; and (3) oxidative stress and LDL susceptibility to oxidation 30 minutes after an acute bout of aerobic exercise. In addition, the relationship between baseline insulin sensitivity, antioxidant status, VO2Max and exercise-induced changes in oxidative stress, CHO and lipid metabolism will be explored. The study will include 35 subjects with type 2 DM (ages 18-40) with duration of diabetes (<7 years) and 35 non-DM controls who will participate in a nonexercise (NE) and an exercise (E) protocols, performed in random order at the General Clinical Research Center. Each of the protocols will be three days in duration. On the first day of either protocol, a glucose clamp will be performed for measurement of insulin sensitivity. On the second day of the E protocol, baseline parameters of oxidative stress, LDL susceptibility to oxidation and lipid metabolism will be obtained prior to an acute bout of aerobic exercise and periodically throughout the post exercise period, during which three meals will be consumed. On the second day of the NE protocol the same protocol will be followed without the acute exercise bout. On the morning of the third day of each protocol (24 hours after the E or NE session), fasting parameters of CHO, lipid and oxidative stress will be drawn. The diet will follow the National Cholesterol Education Program guidelines with 30% fat as energy, with 10:10:10% from saturated, monounsaturated and polyunsaturated fat, respectively with 1/3 of energy requirements consumed at each meal. This study represent a novel approach to the use of aerobic exercise in the treatment of type 2 DM, providing data for future investigations and exercise training studies in this area.