Cooperative Human Tissue Network

本提案的目的是继续在俄亥俄州立大学医学中心作为国家癌症研究所（NCI）的合作人体组织网络（CHTN）的一部分，提供高效高效的组织采购服务。该部门将为美国和加拿大的研究人员管理中央（俄勒冈州立大学医院、詹姆斯癌症医院和研究所、河滨卫理公会医院）和北俄亥俄州（克利夫兰诊所）的采购联盟。作为CHTN的中西部分部，我们将继续提供人类正常、病变、良性和恶性的手术和尸检组织。这包括固体组织、血液/血清、腹水和静脉标本。将对CHTN获得的残余组织进行检查和选择，然后由外科病理学家进行进一步的质量控制和组织学检查。组织将按照研究者指定的指南采购，并在24小时内或根据研究者的需要分批装运。典型的采购方法包括液氮（LN2）快速冷冻组织，OCT介质冷冻组织在LN2冷却的异戊烷中，纱布包裹新鲜组织在冷却的生理盐水中，新鲜组织在运输组织培养基和补充物中，以及福尔马林或gluter醛固定组织。石蜡包埋固定组织也可用。在这项资助中，新的加工组织的替代方法将包括rnase抑制的制备组织，提取肿瘤DNA和RNA，以及石蜡包埋组织基质阵列中肿瘤组的切片。研究者申请将根据CHTN章程优先考虑。该司将继续与中国卫生网络其他司密切合作，在全国范围内联络调查人员的要求，教育研究界了解中国卫生网络标本的可用性，积极参与中国卫生网络议程，以改善服务并制定中国卫生网络范围内的政策和协议。俄勒冈州立大学医学中心正在为患者建立一个全系统的知情同意，允许使用带有结果数据的标本进行研究，或者标本将被断开链接以完全匿名。将继续对标本进行编码，以确保对患者保密。俄勒冈州立大学CHTN的员工包括解剖技术人员，他们在病理学家的指导下接受组织检查技术的全面培训，办公室人员负责数据库录入、更新研究者的要求、分发病理报告、报告统计数据和收集供体相关的医疗信息。此外，俄勒冈州立大学CHTN人员包括一名负责日常操作的病理学家。

The objective of this proposal is to continue to operate a highly productive, efficient tissue procurement service at The Ohio State University Medical Center as a part of the Cooperative Human Tissue Network (CHTN) of the National Cancer Institute (NCI). This division will manage procurement consortiums both in Central (OSU Hospitals, James Cancer Hospital and Research Institute, and Riverside Methodist Hospital) and Northern Ohio (Cleveland Clinic) for research investigators in the United States and Canada. As the Midwestern Division of the CHTN we will continue to provide human normal, diseased, benign, and malignant surgical and autopsy tissues. This includes solid tissue, blood/sera, ascites fluids and pheresis specimens. Remnant tissues obtained for the CHTN will be examined and selected grossly then further quality controlled microscopically with histological review by a surgical pathologist. Tissues will be procured by investigator-specified guidelines and shipped within 24 hours or in batches according to the investigator's needs. Typical methods of procurement include liquid nitrogen (LN2) snap frozen tissues, OCT medium frozen tissue in LN2 cooled isopentane, gauze-wrapped fresh tissues in cooled saline, fresh tissue in transport tissue culture mediums and supplements, and formalin or gluteraldehyde fixed tissues. Paraffin- embedded fixed tissues are also available. In this grant new alternative methods for processed tissue will include RNase-inhibited prepared tissue, extracted tumor DNA and RNA, and slides of tumor sets in paraffin-embedded tissue matrix arrays. Investigator applications will be prioritized according to the CHTN charter. This division will continue to work closely with the other CHTN divisions to network investigator requests nationally, to educate the research community on the availability of CHTN specimens, to actively participate in the CHTN agendas to improve services and develop CHTN-wide policy and protocols. OSU Medical Center is instituting a system-wide informed consent for patients that will allow the use of specimens for research with outcome data or specimens will be delinked for complete anonymity. Specimens will continue to be coded to ensure patient confidentiality. OSU CHTN employees consist of anatomic technologists fully trained and supervised by pathologists in techniques of gross tissue examination, office personnel who perform database entry, update investigator's requests, distribute pathology reports, report statistical data, and gather donor-related medical information. In addition, the OSU CHTN personnel include a pathologist in charge of the daily operations.