Ultrahigh Resolution Optical Barcode

描述(由申请人提供)：基于芯片的分析，如DNA或蛋白质微阵列，其中每个样本通过其在底物上的x，y坐标进行识别，目前具有任何格式中最高的样本密度(样本/面积)。基于颗粒的分析似乎可以提供比平面单层格式(每毫升有1000,000,000个10微米颗粒)高得多的样品密度。但为了接近当前芯片的样本密度，需要某种能够单独区分至少与当前芯片一样多的样本的识别标签或条形码。PARALLEL目前正在开发一种新型的光学编码材料，该材料将被证明在统计上能够在六种颜色系统中以1%的颜色增量解析超过1000万个样本。一种简便、准确、快速的溶液相合成方法将被用来制备含有Dy3+、Er3+、Eu3+、Ho3+、Sm3+和Tm3+发射体的珠子，这些发射体都同时掺入到单一的YVO4基质材料中，可以用单一的紫外光激发源激发。光码由六种颜色的峰值强度之比产生。与有机染料或量子点发出的更宽的发射峰不同，这些基于稀土的材料发射到窄的、不重叠的峰，这些峰可以非常精确地整合，根据不同发射器的相对比例生成独特的光学条形码。为了在有机染料或记者发出与条形码相同的颜色的情况下检测到该条形码，PARALLEL将与BluesShift BioTechnologies合作，利用其非常快速的扫描技术来对有机(激发态寿命约为1 ns)和无机(0.1ms寿命)发射进行时间解析。 该项目建议开发一种新的系统来标记和识别小颗粒。如果能够可靠地识别这些颗粒，它们就可以用来同时跟踪数千种微小的分析。

DESCRIPTION (provided by applicant): Chip based assays such as DNA or protein microarrays, where each sample is identified by its x, y coordinate on the substrate, currently possess the highest sample density (samples/area) of any format. It seems possible that particle based assays could provide far higher sample densities than a planar monolayer format (there are 1000,000,000 ten micron particles/mL). But in order to approach the sample density of the current chips, some sort of identifying label or barcode is needed that is capable of individually distinguishing at least as many samples as current chips. Parallel is currently developing a new type of optical encoding material which will be shown to be statistically capable of resolving over 10,000,000,000 samples at 1% color increments in a six color system. A facile, accurate and rapid solution phase synthesis method will be used to prepare beads containing Dy3+, Er3+, Eu3+, Ho3+, Sm3+, and Tm3+ emitters which are all incorporated simultaneously into a single YVO4 host matrix material which can be excited with a single UV excitation source. The optical code is generated from the ratios of the peak intensities of the six colors. Unlike the broader emission peaks emanating from organic dyes or quantum dots, these rare earth based materials emit into narrow, non-overlapping peaks which can be integrated very precisely to generate a unique optical barcode based on the relative ratios of the various emitters. In order to detect this barcode in the presence of organic dyes or reporters emitting the same color as the barcode, Parallel will collaborate with Blueshift Biotechnologies to employ their very rapid scanning technology to time resolve the organic (excited state lifetime approximately 1ns) and inorganic (0.1ms lifetime) emission. The project proposes to develop a new system to label and identify small particles. If the particles can be reliably identified, they can be used to simultaneously keep track of thousands of miniscule assays.