Arterial Blood Gas Measurement in Small Mammals

描述（申请人提供）：为了保证小型实验动物的生理状态，必须准确和频繁地监测肺和循环功能。动脉血气测量对于这种监测至关重要，然而，重复测量会影响血容量和血细胞比容，特别是在小动物中。该项目将开发试剂和仪器，以持续监测小型哺乳动物外周循环中的动脉血气和pH值。微球，旨在改变其发光发射寿命响应血气和pH值，将被注射到外周动脉循环。将通过测量捕获微球中磷光染料的寿命变化来记录血气参数。该项目开发的微球和仪器将适用于急性研究的动脉血气和pH值的连续监测，以及持续数月的慢性研究的动脉血液参数的定期测量。 第一阶段的研究将开发氧敏感微球，并将测试仪器方法，以测量其磷光性能在现场。这种方法的实验成功将通过测量动脉PO2非侵入性大鼠四肢进行评估。第二阶段的研究将扩展标记微球的能力，以测量pH值和PCO2。

DESCRIPTION (provided by applicant): In order to assure the physiological status of small experimental animals, pulmonary and circulatory functions must be monitored accurately and frequently. Arterial blood-gas measurements are critical for this monitoring, however, repeated measurements can affect blood volume and hematocrit, especially in small animals. This project will develop reagents and instrumentation to continuously monitor arterial blood gases and pH in the peripheral circulation of small mammals. Microspheres, designed to change their luminescent emission lifetimes in response to blood gases and pH, will be injected into peripheral the arterial circulation. Blood-gas parameters will be recorded by measuring lifetime changes of phosphorescent dyes in trapped microspheres. The microspheres and instrumentation developed by this project will be suitable for continuous monitoring of arterial-blood gases and pH for both acute studies and for periodic measurements of arterial-blood parameters for chronic studies lasting several months. Phase I research will develop oxygen-sensitive microspheres and will test instrumental methods to measure their phosphorescent properties in situ. The experimental success of this approach will be evaluated by measuring arterial PO2 noninvasively in the extremities of rats. Phase II research will extend the capabilities of labeled microspheres to measurements of pH and PCO2.