ACTIVITY 3: IMMUNOGENETIC DISEASES TECHNICAL AND MENTORING CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。这项活动的总目标是了解调节免疫和导致哮喘、狼疮和风湿热等疾病中炎症和终末器官损伤的遗传机制，这些疾病对太平洋岛民的影响尤为严重。这一目标将通过以下具体目标来实现：1)建立一个指导环境，帮助青年教师制定创新项目，并培养必要的研究技能，以成功发表和竞争校外资金；2)为资助的发展及资助后的管理提供支持，加强诊所与基础实验室之间的联系，并促进研究成果的及时发表和展示；3)实施假设驱动的发展项目，检查哮喘及其进展到气道重塑（C. Jourdan LeSaux， PI），系统性红斑狼疮及其进展到肾炎（K. Yamamoto， PI），急性风湿热及其进展到风湿性心脏病（D. Kurahara， PI）。在五年的支持期间，该活动在多个地点制定了有效的招募程序，提高了其在基因表达谱和分析方面的技术专长；建立了IgE、链球菌M肽和人心肌肌球蛋白elisa；根据HIPAA和IRB政策优化基因组DNA处理和存档；支持在培养成纤维细胞和过敏小鼠模型中的新发现；并与吉米·埃菲尔德博士一起开始了基于问题的统计学和实验设计培训。临床专业知识在活动中得到充分发展，以进行所有自己的表型表征。我们巩固了与加州大学圣地亚哥分校、德克萨斯大学西南分校、俄克拉荷马大学和约翰霍普金斯大学的研究人员的合作。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The overall goal of this activity is to understand the genetic mechanisms that regulate immunity and contribute to inflammation and end-organ damage in asthma, lupus and rheumatic fever, disorders that disproportionately affect Pacific Islanders. This goal is to be achieved by the following specific aims: 1) establish a mentoring milieu to help junior faculty formulate innovative projects and to develop the necessary research skills to publish and compete successfully for extramural funds; 2) provide support to develop grants and facilitate post-award administration, enhance connectivity between clinics and basic laboratories and promote timely publication and presentation of findings; and 3) implement hypothesis-driven developmental projects that examine asthma and its progression to airway remodeling (C. Jourdan LeSaux, PI), systemic lupus erythmatosus and its progression to nephritis (K. Yamamoto, PI), and acute rheumatic fever and its progression to rheumatic heart disease (D. Kurahara, PI). During the five years of support the activity has developed efficient recruitment procedures at multiple sites, advanced its technical expertise in gene expression profiling and analysis; established IgE, streptococcal M peptide, and human cardiac myosin ELISAs; optimized genomic DNA processing and archiving in compliance with HIPAA and IRB policies; supported new findings in cultured fibroblasts and the allergic mouse model; and initiated problem-based training in statistics and experimental design with Dr. Jimmy Efird. Clinical expertise was fully developed within the activity to conduct all of its own phenotypic characterization. We solidified collaborations with researchers at UCSD, University of Texas Southwestern, the University of Oklahoma and Johns Hopkins University.