Calculating Dynamic CMRO2 Changes Using CBF & BOLD fMRI

描述（由申请人提供）：由神经元活动产生的脑耗氧代谢率（CMR 02）的变化与脑组织功能的能量学直接相关。这些变化有助于血液氧合水平依赖（BOLD）的功能磁共振成像信号，并已部分负责BOLD功能磁共振成像在多学科研究的脑功能的广泛使用。从BOLD fMRI数据中确定CMR02反应的幅度和动力学不仅对解开BOLD反应的基础非常感兴趣，而且对理解大脑功能也非常感兴趣。本提案的目标是确定CMR02的动态变化引起的神经激活使用功能磁共振成像数据，并调查是否瞬时氧运输和消耗的假设是有效的。为此，无创和有创测量CBF、脑血容量（CBV）、组织氧分压（PO2 tiss）。在良好建立的大鼠躯体感觉模型中，使用MRI（9.4特斯拉扫描仪）和非MRI方法获得内在信号的光学成像（OIS）和BOLD测量值。作为该项目的结果，将开发并验证一种使用fMRI数据确定CMR02动态变化的方法。这些方法可用于临床环境中，以评估大脑以及其他器官中的组织活力和功能。

DESCRIPTION (provided by applicant): Changes in the cerebral metabolic rate of oxygen consumption (CMR02) produced by neuronal activity are directly related to the energetics of brain tissue function. These changes contribute to the blood oxygenation level dependent (BOLD) fMRI signal and have been partly responsible for the extensive use of BOLD fMRI in multi-disciplinary studies of brain function. Determining the magnitude and dynamics of CMR02 responses from BOLD fMRI data has been of great interest not only to unravel the underpinning of the BOLD response, but also in the understanding of brain function. The goals of this proposal are to determine the dynamic changes in CMR02 elicited by neural activation using fMRI data and investigate whether the assumption of instantaneous oxygen transport and consumption is valid for this purpose. To this end, non-invasive and invasive measurements of CBF, cerebral blood volume (CBV), tissue oxygen tension (PO2tiss). optical imaging of intrinsic signals (OIS) and BOLD measurements will be acquired using MRI (9.4 Tesla scanner) and non-MRI methodologies in a well-established rat somato-sensory model. As a result of this proposed project, a method to determine the dynamic changes in CMR02 using fMRI data will be developed and validated. These methods can be used in the clinical setting to assess tissue viability and function in the brain as well as other organs.