FLOW CYTOMETRY AND BL/3 CORE FACILITY

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。具体目标：1；巩固和提高两个核心研究支援设施，即生物科学实验所-3和生物科学研究中心核心设施的运作效率。促进和支持本机构聘用的研究妇女健康相关领域的新教员的研究。3. 通过支持Meharry医学院校园内的BSL-3设施，继续为艾滋病毒等传染性病原体的调查提供安全和适当的环境。4. 继续为利用流式细胞术方法研究卵巢癌和乳腺癌的分子生物学以及艾滋病毒感染和其他妇女健康研究领域的研究提供技术基础设施支持。刘斌东博士将担任核心PI，他将为该活动的开展付出10%的努力。刘博士现任BSL-3/FACS核心设施主任，熟悉其操作和维护。他在自己的实验室里操作着一个BSL-2+设备，并拥有HIV-1的研究经验。他将协助教师、博士后和学生发展他们的研究方法，以实现他们的目标，并培训技术人员在BSL-3中使用和应用新的FACS程序和协议开发。研究助理（TBA）：支持高级研究技术员（RA） 100%的工作。RA将接受数据采集和分析方面的培训，用于FACScan、Beckman Coulter Epics XL流式细胞仪和最新设备FACSCalibur的日常操作。他们将协助研究人员进行涉及流式细胞术的项目。RA将能够协助用户进行任何细胞分选，作为艾滋病毒和艾滋病相关传染病研究或其他需要细胞分选技术的项目的一部分。作为一名高技能人员，RA也将接受BSL-3程序的培训，并将协助和培训使用该设施的个人。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. SPECIFIC AIMS: 1. To consolidate and improve the efficiency of operation of two core research support facilities, the BSL-3 and the FACS Core Facility. 2. To facilitate and support the research of new faculty recruited to the institution who study areas of concern in women¿s health. 3. To continue to provide a safe and appropriate environment for the investigation of infectious agents such as HIV by supporting the BSL-3 facility on the Meharry Medical College campus. 4. To continue to provide technological infrastructure support to research utilizing methods in flow cytometry in the investigation of molecular biology of ovarian and breast cancer, as well as HIV infection and other areas of women¿s health research. Bindong Liu, Ph.D., will be the Core PI and he will commit 10% effort to the conduct of this activity. Dr. Liu is the current Director of the BSL-3/FACS Core Facility and is familiar with the operation and maintenance of it. He operates a BSL-2+ facility in his laboratory and has research experience with HIV-1. He will assist faculty, post docs and students develop their research methodology to achieve their objectives as well as training the technician on the use and application of new FACS procedures and protocol development in the BSL-3. Research Assistant (TBA): Support for 100% effort of a Senior Research Technician (RA). The RA will be trained in data acquisition and analysis for day-to-day operation of the FACScan, Beckman Coulter Epics XL Flow Cytometer and the newest addition to the facility, the FACSCalibur. They will assist investigators with projects involving flow cytometry. The RA will be able to assist users in any cell sorting required as part of the HIV and AIDS-related infectious disease research or other projects requiring the cell sorting technology. As a highly skilled individual, the RA will also be trained in BSL-3 procedures and will be expected to assist and train individuals who use that facility.