CELL CULTURE CORE FACILITY

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。在培养中保持哺乳动物和其他真核细胞的能力是研究细胞免疫和生理反应以及研究病毒和结核分枝杆菌等感染性病原体的重要组成部分。需要专门的培养设备和孵化器来保持这种培养。这项提议为研究人员提供了一个可访问的、可管理的核心设施，为边境生物医学研究中心的研究人员提供设备和技术支持，这些研究人员的项目依赖于细胞培养。设备基础设施将包括生物安全柜、二氧化碳孵化器、冷藏和超低温储存设施、倒置和复合显微镜以及超纯水供应。这种支持对于研究毒物对细胞的影响、细胞免疫反应以及对乙肝病毒和分枝杆菌的研究是必不可少的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ability to maintain mammalian and other eukaryotic cells in culture is an essential part of studies on cellular immune and physiological responses as well as investigations into infectious agents such as viruses and Mycobacterium tuberculosis. Specilized culturing equipment and incubators are required to maintain such cultures. This proposal provides for an investigator accessible, managed core facility to provide equipment and technical support for Border Biomedical Recearch Center researchers whose projects rely on cell culture. Equipment infrastructure will include hiosafety cabinets, CO2 incubators, deep freeze and ultra low temperature storage facilities, inverted and compound microscopes, and ultrapure water supply. Such support is and will be essential for the support of research into the effects of toxicants on cells, cellular immune responses, and studies on HBV and mycobacteria.