Linear Ion Trap Mass Spectrometer

描述(由申请人提供)：我们建议购买一台ThermoFinnigan LTQ线性离子陷阱质谱仪，以放置在现有的眼科蛋白质组学和质谱学核心中。之所以需要这种仪器，是因为目前在核心使用的LCQ Classic离子陷阱不再满足正在进行的项目的需要，这些项目需要更高灵敏度和更快的数据采集速度的仪器。 新仪器将支持眼科核心赞助项目以及俄勒冈健康与科学大学多个合作项目的正在进行的研究。该仪器将专门用于多肽消化的纳米电喷雾电离分析，以产生用于蛋白质鉴定的串联质谱数据。现有的安捷伦毛细管液相色谱系统将与该仪器一起使用，以便在高通量环境中进行自动化分析。目前的经典LCQ将专门用于大气压电离MALDI源，用于分析电泳胶中的简单消化。将每台仪器专门用于电喷雾或MALDI分析，将极大地提高岩芯的效率和生产率。 拟议的线性陷阱的灵敏度和扫描速度的提高将用于蛋白质/蛋白质相互作用研究中的复杂多肽消化，以及组织匀浆和体液中蛋白质的鸟枪式鉴定和定量。拟议的使用多维层析的实验是可行的，因为核心雇用了两名全职生物信息学专家和一名实验室助理，目前正在进行这些类型的实验，并拥有一套处理大型数据集的软件工具和硬件。 这一最先进的仪器将用于11个不同的多学科项目，研究白内障、青光眼、葡萄膜炎、视网膜炎、角膜损伤、囊性纤维化、肾病综合征、暴露于毒素后的中枢神经系统功能障碍、细胞凋亡抑制和胶质母细胞瘤的分子病因。另一个用途将是研究人类唾液中蛋白质的组成，以测试这种液体在检测疾病生物标志物方面的有用性。

DESCRIPTION (provided by applicant): We propose the purchase of a ThermoFinnigan LTQ Linear Ion Trap Mass Spectrometer to place in an existing Ophthalmology Proteomics and Mass Spectrometry Core. This instrument is required because the LCQ Classic ion trap now used in the core no longer fulfills the needs of the on-going projects that require an instrument of greater sensitivity and rate of data acquisition. The new instrument will support the on-going research of both Ophthalmology Core sponsored projects, and a number of collaborative projects throughout Oregon Health & Science University. The instrument will be exclusively used for nano-electrospray ionization analysis of peptide digests to generate tandem mass spectral data for protein identification. An existing Agilent capillary liquid chromatography system will be used with the instrument to perform automated analysis in a high throughput environment. The current LCQ classic will be dedicated for use with an atmospheric pressure ionization MALDI source for analysis of simple digests from electrophoresis gels. Dedicating each instrument for either electrospray or MALDI analysis will greatly increase the efficiency of the core and increase productivity. The increased sensitivity and scan rates of the proposed linear trap will be used for complex peptide digests derived from protein/protein interaction studies and shotgun identification and quantification of proteins from tissue homogenates and body fluids. The proposed experiments using multidimensional chromatography are feasible because the core employs two full time bioinformatics specialists and a laboratory associate currently performing these types of experiments, and possessing a suite of software tools and hardware to handle the large data sets. This state-of-the-art instrument will be used in 11 diverse multi-disciplinary projects that will study the molecular causes of: cataract, glaucoma, uveitis, retinitis, corneal injury, cystic fibrosis, nephrotic syndrome, central nervous system dysfunction following exposure to toxins, suppression of apotosis, and glioblastomas. An additional use will be to study the composition of proteins in human saliva to test the usefulness of this fluid to detect biomarkers for disease.