Multianalyte Nanomagnetic Biosensor Array for Pathogen Detection

描述(申请人提供)：该SBIR第一阶段项目旨在将巨磁电阻(GMR)传感器设备改造成用于病原体检测的自旋电子式多分析物生物传感器。从医学诊断到打击生物恐怖主义，生物传感器技术的潜在价值正在被广泛的应用所认识。该项目将专注于开发GMR免疫分析方法，以检测供水中的常见细菌和原生动物。磁性纳米/微米颗粒作为生物分子结合的标记物具有稳定性好、背景信号低等优点。该项目将为NVE公司正在开发的GMR微电极阵列和海军研究实验室正在开发的顺磁微珠作为珠阵列计数器(BARC)生物传感器系统的标签的生物传感器应用提供改进的生物分子耦合技术。第一阶段的工作将包括光化学和电化学聚合物衍生物的合成和使用测试，以分别将单克隆和多克隆抗体结合到氮化硅表面和磁性颗粒上。信号/噪声特性将通过荧光和磁性分析方法进行测量，并被证明与当前使用的分析方法相同或更好。这些第一阶段的结果有望为优化第二阶段的生物分子固定化化学提供坚实的技术背景，以及用于增强GMR生物传感器流动单元的微流体通道涂层和盖子粘附性的新型但相关的潜伏性-反应性聚合物。开发一种实用、低成本、便携式的GMR病原体生物传感器将有助于人类健康，因为它可以快速分析现场的当地供水情况，从而最大限度地减少腹泻感染。

DESCRIPTION (provided by applicant): This SBIR Phase I project is designed to adapt a Giant Magneto-Resistive (GMR) sensor device into a "spintronic" multianalyte biosensor for pathogen detection. The potential value of biosensor technology is being recognized for a wide variety of applications, from medical diagnostics to countering bioterrorism. This project will focus on developing GMR immunoassays to detect common bacteria and protozoa in water supplies. Magnetic nano/microparticles present advantages as labels for biomolecule binding, including stability and low background signal. This project will provide improved biomolecule coupling technology for biosensor applications of the GMR microelectrode array under development by NVE Corp. and the paramagnetic microbeads under development as labels by the Naval Research Laboratories for the Bead Array Counter (BARC) biosensor system. The Phase I effort will include the synthesis and use testing of photochemical and electrochemical polymer derivatives for binding monoclonal and polyclonal antibodies to the silicon nitride surface and magnetic particles respectively. Signal/noise properties will be measured by fluorescent and magnetic assay methods and shown to be equal or superior to currently used assays. These Phase I results are expected to provide a solid technical background for optimizing the biomolecule immobilization chemistry in Phase II, as well as new, but related latent-reactive polymers for enhanced microfluidic channel coating and lid adhesion for the GMR biosensor flow cell. Development of a functional low cost, portable GMR biosensor for pathogens will aid human health by allowing quick analysis of local water supplies in the field, thereby minimizing diarrheal infections.