Zebrafish Wound Healing Model for Drug Screening

描述（由申请方提供）：慢性伤口是一种严重的健康问题，随着人口老龄化和糖尿病患者数量的显著增加而加剧。缓慢或受损的伤口愈合对于免疫抑制或卧床不起的个体也是一个问题。血管生成过程中的缺陷与伤口愈合缓慢或不愈合有关。虽然刺激慢性伤口中的血管生成已被证明可以加速伤口愈合，但该领域的药物发现一直很缓慢。用这种方法开发有效治疗剂的主要障碍之一是常规的体内测定，如小鼠和大鼠伤口愈合模型，是昂贵的和劳动密集型的。该SBIR旨在开发一种体内斑马鱼模型，以筛选可以通过刺激血管生成过程来改善伤口愈合的药物。该SBIR将研究胚胎发生、图案形成、伤口愈合率和伤口愈合过程中的血管生成反应。|ing.斑马鱼伤口愈合模型将有助于简化药物发现过程。

DESCRIPTION (provided by applicant): Chronic wounds represent a serious health problem, exacerbated by the aging population and a significant increase in the number of diabetic patients. Slow or impaired wound healing is also a problem for immunosuppressed or bedridden individuals. Defects in the angiogenesis process are associated with slow or absence of wound healing. Although stimulating angiogenesis in chronic wounds has been shown to accelerate wound healing, drug discovery in this area has been slow. One of the major hurdles in developing effective therapeutics with this approach is that conventional in vivo assays, such as mouse and rat wound healing models, are costly and labor intensive. This SBIR aims to develop an in vivo zebrafish model to screen drugs that can improve wound healing by stimulating the process of angiogenesis. This SBIR will investigate embryogenesis, patterning, wound healing rate and angiogenic response during wound hea|ing. A zebrafish wound healing model will assist in streamlining the drug discovery process.