4000 Q TRAP triple quadrupole/linear ion trap LC/MS/MS

描述（由申请人提供）：本NCRR共享仪器提案旨在为购买应用生物系统4000 Q-TRAP HPLC-MS三重四极杆线性离子阱质谱仪（带纳米电喷雾电离源）寻求资金。该集成平台将包括一个Dionex在线纳米毛细管HPLC系统，配有自动进样器和用于样品富集的开关装置。Q-TRAP质谱仪将配备Turbo V源、TurbolonSpray探头、NanoSpray源和接口。还要求提供数据采集和数据处理所需的全套仪器软件，以支持本提案中所述的研究目标。简而言之，我们建议将该仪器几乎完全用于在线纳米HPLC-MS/MS，用于分离和分析复杂的蛋白质/肽混合物。这些分析将强调4000 Q TRAP的高灵敏度和特殊扫描功能，包括多反应监测、前体离子和中性损失扫描功能。这些独特的能力将针对生物学上重要的酶和非酶翻译后修饰，包括蛋白质磷酸化，氧化损伤和亚硝基化的广泛阵列的识别和定量。主要但不是唯一的重点将是支持巴克研究所的一系列NIH资助的项目，这些项目旨在了解衰老和年龄相关疾病的分子基础，因为它们与生物学相关的翻译后修饰的变化相关。

DESCRIPTION (provided by applicant): This NCRR shared instrumentation proposal seeks funding for the purchase of an Applied Biosystems 4000 Q-TRAP HPLC-MS triple quadrupole linear ion-trap mass spectrometer with nano-electrospray ionization source. This integrated platform will include a Dionex on-line nano-capillary HPLC system with autosampler and switching device for sample enrichment. The Q-TRAP mass spectrometer will be equipped with a Turbo V Source, a TurbolonSpray probe, a NanoSpray Source and Interface. A full complement of instrument software required for data acquisition and data processing is also requested to support the research aims described in this proposal. In brief, we propose to operate this instrument almost exclusively (>95% use) for on-line nano HPLC-MS/MS for the separation and analysis of complex protein/peptide mixtures. These analyses will emphasize the high sensitivity and special scan features of the 4000 Q TRAP, including multiple reaction monitoring, and precursor ion and neutral loss scanning functions. These unique capabilities will be directed at the identification and quantitation of a wide array of biologically important enzymatic and non-enzymatic posttranslational modifications, including protein phosphorylation, oxidative damage and nitrosylation. The primary, but not exclusive focus, will be on supporting a wide array of NIH-funded projects at the Buck Institute that seek to understand the molecular basis for aging and age-related diseases as they correlate to changes in biologically relevant posttranslational modifications.