An LTQ-FT Mass Spectrometer for Shotgun Proteomics

描述（由申请人提供）：所附提案要求通过购买ThermoFinnigan LTQ-FT质谱仪，为华盛顿大学的质谱和蛋白质组学资源提供资金。该仪器将是非常宝贵的成功完成我们现有的NIH资助的项目，涉及定性和定量分析的蛋白质和蛋白质修饰的复杂混合物。此外，我们的一些项目正在将定量蛋白质组学技术扩展到测量蛋白质表达和降解速率的变化（蛋白质动力学）。及时完成这些NIH研究项目的必要条件是质谱仪的可用性，该质谱仪能够i）低于2 ppm的质量精度以区分类似质量的肽，ii）在单个谱中>1000的动态范围，iii）具有四极离子阱的灵敏度和速度的串联质谱的数据依赖性采集，和iv）分辨和检测复杂混合物中多电荷肽的异常同位素分布的能力。华盛顿大学的研究人员没有这样的共享仪器，我们的几个用户前往外部实验室完成他们的NIH资助项目。虽然我们已经设法解决了缺乏能够进行这些测量的质谱仪的问题，但如果我们希望保持生产力和竞争力，我们就不能继续这样做。我们建议解决这些限制，通过收购一个共享的混合质谱仪相结合的鲁棒性和速度的线性四极离子阱的分辨率，质量精度和动态范围的傅里叶变换离子回旋质谱仪。所要求的仪器的性能将允许教师在全球范围内解决当前的技术限制，并将提供大学内不可用的资源。

DESCRIPTION (provided by applicant): The enclosed proposal is requesting funds to expand the mass spectrometry and proteomics resources at the University of Washington through the purchase of a ThermoFinnigan LTQ-FT mass spectrometer. This instrument will be invaluable in the successful completion of our existing NIH funded projects involving the qualitative and quantitative analysis of proteins and protein modifications of complex mixtures. Furthermore, a number of our projects are extending quantitative proteomics technology to the measurement of changes in the rate of protein expression and degradation (protein dynamics). Integral to the timely completion of these NIH research projects is the availability of a mass spectrometer capable of i) sub 2 ppm mass accuracy to distinguish between similar mass peptides, ii) a dynamic range of >1000 in a single spectrum, iii) data-dependent acquisition of tandem mass spectra with the sensitivity and speed of a quadrupole ion trap, and iv) the ability to resolve and detect unusual isotope distributions of multiply charged peptides within a complex mixture. No such shared instrument is available to investigators at the University of Washington and several of our Users travel to outside laboratories to complete their NIH funded projects. Although we have managed to work around the lack of availability of a mass spectrometer capable of these measurements we cannot continue in this way if we hope to remain productive and competitive. We propose to address these limitations through the acquisition of a shared hybrid mass spectrometer which combines the robustness and speed of a linear quadrupole ion-trap with the resolution, mass accuracy, and dynamic range of a Fourier transform ion cyclotron mass spectrometer. The performance of the requested instrumentation will allow the faculty to address current technological limitations on a global level and will provide a resource that is not available within the university.