FLOW CYTOMETRY FACILITY HIGH SPEED SORTER

描述（由申请人提供）：为了升级Thomas Jefferson大学Kimmel癌症中心的多用户流式细胞仪设备，申请获得一台具有分选能力的最先进的流式细胞仪，从而最佳地支持美国国立卫生研究院资助的癌症中心成员的研究活动。我们要求购买一台具有高速四路分选能力的Cytomation MoFlo(TM) HTS超高速分选流式细胞仪，可常规同时分析4种以上的荧光染料，并具有单细胞沉积能力。目前，KCC流式细胞仪设备是TJU校园内唯一的此类设备。它配备了一台使用了12年的库尔特EPICS Elite ESP（也是一种分选仪器）和两台分析仪：一台使用了4年的库尔特EPICS XL-MCL和一台使用了15年的Becton-Dickinson FACSCalibur台式分析仪。新获得的BD咏叹调分拣机在TJU神经内科实际存在（尽管尚未安装在新开发的设施中）。然而，无论何时在操作中，这台设备都将专门用于神经科学研究的特定需求，KCC调查员的访问权限有限。越来越多的KCC研究人员在他们的nih资助的项目中需要高速细胞分选，这是我们在现有和正在运行的KCC设施中要求使用细胞化MoFlo的主要理由。我们的申请集中在这个核心设施升级其服务的需求上，因为：1)我们的美国国立卫生研究院研究人员对在合理的时间内分类样品的理想细胞产量的要求越来越高，这是目前使用现有设备无法实现的；2)在大细胞群中进行罕见事件分选的需求日益增加，同时分析超过4个荧光染料可检测参数的需求日益增加；3)缺乏足够可靠的就近高速分拣服务。所要求的细胞化MoFlo仪器的可用性将允许在KCC继续进行出色的基础和临床研究，并启动新的项目。它还将提高现有设施的效率，使更多的调查人员能够可靠地使用仪器，并使其他调查人员（不需要这里要求的机器）能够及时地在旧设备上进行更常规的分析和分类，而不会出现目前存在的滞后现象。

DESCRIPTION (provided by applicant): This proposal is to acquire a state-of-the-art flow cytometer with sorting capabilities in order to upgrade the multi-user Flow Cytometry Facility of the Kimmel Cancer Center, Thomas Jefferson University, and thus optimally support the research activities of the NIH-funded members of the Cancer Center. We request to purchase a Cytomation MoFlo(TM) HTS Ultra High-Speed Sort Flow Cytometer with capability of high speed 4-way sorting, of routine simultaneous analysis of greater than 4 fluorochromes, and with the added capability of single cell deposition. Currently, the KCC Flow Cytometry Facility is the sole facility of this type on the TJU campus. It is equipped with a 12-year old Coulter EPICS Elite ESP, which is also a sorting instrument, and two analyzers: a 4-year old Coulter EPICS XL-MCL, and a 15- year old Becton-Dickinson FACSCalibur Benchtop Analyzer. A newly acquired BD Aria Sorter is physically present (although yet to be installed in a newly to-be-developed facility) in the Neurology Department at TJU. Whenever in operation, however, this piece of equipment will be dedicated to the specific needs of Neurological Sciences research, with the KCC investigator having limited access. The increased number of KCC investigators requiring high-speed cell sorting in their NIH-sponsored projects serves as the main justification for our request for a Cytomation MoFlo in the existing and operating KCC facility. Our application is focused on the need of this core facility to upgrade its services as a result of: 1) our NIH-investigators' increasing requests for desirable cell yields of sorted samples in reasonable time periods, which are currently unachievable with the present equipment; 2) an increasing demand for rare-event sorting within large cell populations, and for simultaneous analysis of greater than 4 fluorochrome-detectable parameters; and 3) the lack of sufficient and reliable access to near-by high speed sorting services. The availability of the Cytomation MoFlo instrument requested would allow outstanding basic and clinical research to be continued, and new projects to be initiated, at the KCC. It also would increase the efficiency of the existing Facility, allowing reliable use of the instruments by more investigators, and by other investigators (not in need of the machine requested here) to perform the more routine analysis and sorting in a timely manner on the old equipment, without the lag existent at present.