ATOMIC FORCE MICROSCOPE: MAGNETIC FORCE MICROSCOPY

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：戴维斯心肺研究所（DHLRI）正在申请原子力显微镜（AFM）系统，以在俄亥俄州立大学（OSU）医学和公共卫生学院（COMPH）建立AFM核心设施。所要求的仪器是来自digital Instruments的Bioscope AFM系统。该系统将由数字仪器公司的Nanoscope IV控制器、BioScope AFM、用于数据分析的离线工作站、蔡司倒置光学显微镜Axiovert 200以及一些必要的AFM附件和图像处理软件组成。俄勒冈州立大学的COMPH和其他联合生物医学科学学院没有任何原子力显微镜仪器。目前，我们只能使用俄勒冈州立大学西校区工程学院的升级版多模AFM，该AFM带有纳米范围的Ilia控制器。除了物理上远离COMPH之外，这种AFM在样本量和有限的技术能力方面也有限制，只有像Bioscope这样的仪器才能克服这些限制。Bioscope对于适应俄勒冈州立大学的研究项目的数量和多样性至关重要，这些项目将受益于使用AFM技术。这份获得最先进的AFM系统的提案是代表3个获得NIH资助的主要用户和4个获得NSF或其他资助的次要用户提交的。为了满足不同用户群体的AFM需求，需要一种最先进的AFM仪器，它既能完成高分辨率的纳米级成像，又能同时对细胞样本和组织切片进行光学显微镜检查。NIH项目将使用所要求的Bioscope AFM来研究细胞骨架、胞外分泌和细胞外基质调节、组织切片的地形和表面表征。这些研究将有助于回答纳米级的生物学问题，这些问题将与NIH项目中使用大量其他技术获得的数据相关。AFM的新生物应用，如磁力显微镜（MFM）和浸笔纳米光刻（DPN），将成为AFM核心设施创新研究和开发的新载体。DHLRI和大学为相关实验室设施的发展提供了支持，并将以员工工资的形式提供持续的机构支持。获得该AFM仪器将极大地增强主要用户的研究计划，并帮助俄勒冈州立大学的其他生物医学研究人员。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Davis Heart and Lung Research Institute (DHLRI) is requesting an atomic force microscope (AFM) system to establish an AFM core facility at the Ohio State University (OSU) College of Medicine and Public Health (COMPH). The requested instrument is a Bioscope AFM system from Digitial Instruments. The system will be comprised of a Digital Instrument's Nanoscope IV controller, a BioScope AFM, an offline workstation for data analysis and the Zeiss inverted light microscope Axiovert 200 along with a number of necessary AFM accessories and the image processing softwares. The COMPH and other allied Colleges of Biomedical Sciences at OSU do not have any AFM instrumentation. Our current access is limited to an upgraded MultiMode AFM with a Nanoscope Ilia controller in the College of Engineering, a considerable distance away at the West Campus of OSU. Besides being physically away from the COMPH, this AFM has restrictions on the sample size and limited technical capabilities which can only be overcome by an instrument like the Bioscope. The Bioscope is essential to accommodate the quantity and diversity of the research projects at OSU that will benefit by using the AFM technology. This proposal for acquiring a state-of-the art AFM system is being submitted on behalf of 3 major users with NIH funding and 4 minor users with NSF or other funding. To fulfill the diverse AFM needs of the user group, a state-of-the-art AFM instrument is needed which can accomplish both high-resolution nanoscale imaging and a simultaneous light-microscopy examination of the cell samples and tissue sections. The NIH projects will use the requested Bioscope AFM for investigating cell cytoskeleton, exocytosis and extracellular matrix regulation by cells, topography of tissue sections and surface characterization. These investigations will help in answering biological questions at the nanoscale level which will then be related to the data acquired using a multitude of other techniques in the NIH projects. Novel biological applications of AFM like magnetic force microscopy (MFM) and dip-pen nanolithography (DPN) will form a new vector of innovative research and development of the proposed AFM core facility. DHLRI and the University have provided support for the related laboratory facility development and will provide continuing institutional support in the form of staff salary. Acquisition of this AFM instrumentation will greatly enhance the research programs of the major users and aid other biomedical investigators at OSU.