4000 Q TRAP LC/MS/MS SHARED MASS SPECTROMETER: PROSTATE &OVARIAN CANCER,LEUKEMIA

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：弗吉尼亚联邦大学有一个非常活跃和不断增长的资助研究人员的临界质量，致力于了解生物活性脂质的动态变化如何影响代谢和调节正常和病理人类细胞功能的信号通路。申请资金用于应用生物系统4000 Q TRAP(tm) LC/MS/MS系统，这是一种混合三重四极杆/线性离子阱质谱联用液相色谱仪。这种异常灵活的仪器结合了最灵敏的三重四极杆质谱仪的独特功能，可提供最高容量离子阱的质量分辨率，灵敏度和动态响应所需的结构表征和定量组成脂质组的各种分子。如本应用程序所述，来自7个不同部门的18名NIH资助的科学家和临床医生参与了超过45个NIH支持的研究项目，他们对该质谱仪有特定的需求。4000 Q TRAP将作为一个共享的研究资源，为我们正在进行的研究项目提供必要的支持，并促进新的脂质鉴定、定量和表征的合作研究计划，特别强调细胞信号传导和功能性脂质组学。提供这一要求的主要理由是，医疗中心校园内没有具备所需功能的质谱仪。该仪器将在Sarah Spiegel和William M. Grogan教授的指导下作为VCU核心资源进行管理，他们在脂质分析和核心资源管理方面拥有丰富的经验。Richard Moran教授将担任咨询委员会主席，监督该工具的公平使用，以造福受资助的研究人员和大学的研究环境。购买这台质谱仪对继续发展和实现脂质组学研究的全部潜力至关重要，以更好地了解VCU医学中心校园内的正常细胞功能和人类疾病。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Virginia Commonwealth University has a very active and growing critical mass of funded investigators working to understand how dynamic changes in bioactive lipids influence metabolism and the signaling pathways that regulate normal and pathological human cellular functions. Funds are requested for an Applied Biosystems 4000 Q TRAP(tm) LC/MS/MS System, a hybrid triple quadrupole/linear ion trap mass spectrometer coupled with a liquid chromatograph. This exceptionally flexible instrument combines the unique capabilities of the most sensitive triple quadrupole mass spectrometer available with the highest capacity ion trap to provide mass resolution, sensitivity, and dynamic response needed for structural characterization and quantitation of the diverse molecules that comprise the lipidome. As described in this application, 18 NIH-funded scientists and clinicians from 7 different departments involved in over 45 NIH- supported research programs have specific needs for this mass spectrometer. The 4000 Q TRAP will be operated as a shared research resource to provide essential support for our ongoing research programs and to facilitate new collaborative research initiatives for lipid identification, quantitation, and characterization, with particular emphasis on cell signaling and functional lipidomics. Providing a principal justification for this request, there is no mass spectrometer with the required capabilities on the Medical Center campus. The instrument will be administered as a VCU core resource under the direction of Professors Sarah Spiegel and William M. Grogan, who have extensive experience in lipid analysis and administration of core resources. Prof. Richard Moran will chair the Advisory Committee overseeing equitable use of the instrument for the benefit of funded investigators and the university research environment. The purchase of this mass spectrometer is essential to continuing development and realization of the full potential of lipidomics research toward better understanding of normal cellular function and human disease on the VCU Medical Center campus.