BECTON DICKENSON FACSARIA CELL SORTER: IMMUNOLOGY

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：我们正在申请资金购买Becton Dickenson FACSAria高速细胞分选机。所要求的配置包括三个激光器，能够检测多达九种具有一系列激发和发射特性的不同荧光色。该仪器还包括单个分选细胞的收集和温度控制功能。新仪器将用于支持美国国立卫生研究院资助的项目的许多分析和分类应用。这些应用包括细胞信号分子的多路免疫荧光分析，从动物组织中分选白细胞，以及高亲和力抗体和多肽的酵母展示文库筛选。新仪器将被安置在LJBI流式细胞仪设备中。已经制订了进入和使用的政策，并已作出体制承诺，为最初三年的业务提供人员和维修方面的支助。FACSAria在LJBI先进的流式细胞仪开发和应用能力的发展中起着关键作用。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We are requesting funds to purchase a Becton Dickenson FACSAria high speed cell sorter. The requested configuration includes three lasers, enabling the detection as many nine different fluorochromes with a range of excitation and emission properties. The instrument also includes capabilities for the collection and temperature control of single sorted cells. The new instrument will be used for a number of analysis and sorting applications in support of NIH-funded projects. These applications include multiplexed immunofluorescence analysis of cell signaling molecules, sorting of leukocytes from animal tissue, and the screening of yeast display libraries for high affinity antibodies and polypeptides. The new instrument will be housed in the LJBI Flow Cytometry Facility. Policies for access and use have been formulated, and an institutional commitment is in place to support personnel and maintenance for the initial three years of operation. The FACSAria plays a key role in the development of an advanced flow cytometry development and application capability at LJBI.