Core C: Analytical Core

分析化学核心的总体目标是为 计划项目中的人体监测、动物模型和细胞生物学项目。我们会申请 适当地向研究项目提供技术，并将促进 分析方法论是基于。该核心将对来自 上一次资助期内收集的费用研究，从按存储容量使用计费中获得的新样本 和弹珠研究(见项目1和2)。我们将评估新方法以应用于 对未来兄弟姐妹的前瞻性研究，并检验特定的假设。一个主要目标将是产生 外源暴露和代谢生物标记物的数据集，以对抗转录组和 通过确定外来生物暴露和内源代谢物水平来获得蛋白质组数据 已选择。我们将评估强调免疫功能障碍的动物模型中的代谢物分布。 在人类样本中，我们将特别注意指示炎症状态的代谢物 最近发现血浆瘦素水平是区分早发性和非早发性疾病的生物标志物 临床退化型自闭症。我们还将监测T细胞、B细胞和自然细胞中的代谢物 正常儿童和自闭症儿童的杀伤(NK)细胞，有无暴露于异物。平等的 重要的是，我们将在分析化学领域提供步进式仪器和咨询服务 具体目标的整个核心是： 1.建立导致自闭症患者血脂成分变化的代谢途径 儿童及其兄弟姐妹之间以及正常和免疫挑战的动物模型之间 自闭症。 2.使用全球代谢组学程序寻找自闭症的生物标记物。 3.提供关于细胞和活体模型系统中农药和其他外来生物水平的分析数据 并在血清样本中进行充值、充值、弹珠等后继 学习。 4.开发计划项目中科学家感兴趣的外来生物的新分析方法。

The overall objective of the Analytical Chemistry Core is to provide analytical support for the human monitoring, animal model, and cell biology projects in the program project. We will apply techniques to the research projects as appropriate, and will advance the technology upon which the analytical methodology is based. This core will conduct new analyses on serum samples from the CHARGE study, collected during the previous funding period, new samples from the CHARGEBACK and MARBLES study (see Projects 1 and 2). We will evaluate new methods to apply to the prospective study of future siblings and to test specific hypotheses. A major goal will be to generate data sets of xenobiotic exposure and metabolomic biomarkers to regress against transcriptome and proteome data by determining both xenobiotic exposure and levels of endogenous metabolites on selected . We will evaluate metabolite profiles in animal models emphasizing immune dysfunction. In human samples, we will pay particular attention to metabolites indicative of inflammatory status and plasma leptin levels recently found as a biomarker to distinguish between early onset and clinical regression autism. We also will monitor the metabolite profiles in T cells, B cells and natural killer (NK) cells of normal and autistic children with and without exposure to xenobiotics. Of equal importance we will provide a walk up instrument and consultation service in analytical chemistry for the entire core the Specific Aims are: 1. Establish the metabolic pathways responsible for variations in lipid composition between autistic children and their siblings and between normal and immunologically challenged animal models of autism. 2. Use global metabolomic procedures to search for biomarkers of autism. 3. Provide analytical data on pesticide and other xenobiotic levels in cell and in vivo model systems and in serum samples from the CHARGE, CHARGE-BACK, MARBLES, and other successor studies. 4. Develop new analytical methods for xenobiotics of interest to scientists in the program project.