Core--Multiplexed Gene Analysis

多重基因分析（MGA）核心目前为癌症中心成员提供高通量基因表达分析技术。这些技术往往与相当大的基础设施、成本、质量控制和/或复杂性有关，因此难以在单个实验室建立。该MGA核心提供基因表达谱服务使用Affyscore基因芯片？寡核苷酸微阵列服务包括从标准（3-5 μ g）和小规模（10-30 ng）RNA样品进行阵列杂交和标记靶制备。阵列数据直接集成到SCC生物信息学核心，其中提供数据检索和数据挖掘工具。特别强调数据质量保证和样本跟踪。MGA Core还提供定量（实时）RT-PCR服务。研究者可以选择准备自己的检测试剂盒，然后提交给核心进行分析。或者，使用市售的、预先验证的引物组， Core将使用研究者提供的RNA执行基因表达试验的所有步骤。未来，基于阵列和基于PCR的SNP检测将在Core现有的技术平台上得到支持。在过去的一年里，核心已经为40多个癌症中心成员项目生成了基因表达数据，因此继续成为基础和转化癌症研究的重要技术资源。

The Multiplexed Gene Analysis (MGA) Core currently provides Cancer Center members with access to technologies for high-throughput gene expression analysis. These technologies often are associated with considerable infrastructure, cost, quality control and/or complexity, making them difficult to establish in individual laboratories. The MGA Core provides gene expression profiling services using Affymetrix GeneChip ?oligonucleotide microarrays. Services include array hybridization and labeled target preparation from standard (3-5 ug) and small scale (10-30 ng) RNA samples. Array data is directly integrated into the SCC Bioinformatics Core, where data retrieval and data mining tools are made available. Particular emphasis is placed on data quality assurance and sample tracking. The MGA Core also provides quantitative (real-time) RT-PCR services. Investigators may elect to prepare their own assays that are then submitted to the Core for analysis. Alternatively, using commercially available, pre-validated primer sets, the Core will perform all steps of the gene expression assay with RNA provided by the investigator. In the future, array-based and PCR-based SNP assays will be supported on the Core's existing technology platforms. Within the past year, the Core has generated gene expression data for over forty cancer center member projects, thus continuing to be a significant technical resource for both basic and translational cancer research.