Novel Multimodal Selectors for CE and HPLC

描述（由申请人提供）：目前，高效液相色谱分离发展最快的领域是生物分子分离和液相色谱-质谱（LC-MS）领域。虽然在过去的几十年里，质谱分析取得了惊人的进步，但与质谱仪相结合的分离中固有的不足仍然对访问生物样品中的全部信息内容构成了相当大的障碍。生物分子分离是具有挑战性的，因为它们呈现出从疏水到极性到静电的各种相互作用模式。拟议的工作解决了迫切需要改进的技术，用于生物聚合物的新型和更通用的分离策略（例如，多肽、蛋白质、寡核苷酸），同时能够表征溶质/离子液体相互作用。提出的工作的目的是将新兴的离子液体领域的生物分子分离的新的分离策略，其目标是提供一种快速的方法，用于评估离子液体/目标分析物的相互作用，一类新的固定相，使复杂的生物分子混合物的分离。 我们假设，建议的选择器将提供多种多样的生物分子分析物的分离的多模式保留。 两个具体目标将解决这一假设： 1.离子液体选择剂的毛细管电泳研究毛细管电泳将用于快速筛选离子液体/选定的分析物相互作用。我们将迁移数据与离子液体/分析物亲和力相关。 2.离子液体选择剂的液相色谱研究保留数据将在建议的HPLC吸附剂上获得，从小分子（例如，芳香族化合物、核苷酸、氨基酸）并延伸到更大的分子（例如，肽、多核苷酸）作为这种新介质所包含的分离空间。

DESCRIPTION (provided by applicant): Currently, the fastest growing areas of high performance liquid chromatographic separations are in the areas of biomolecule separations and liquid chromatography-mass spectrometry (LC-MS). While the last few decades have seen phenomenal progress in mass spectrometry, the shortfalls inherent in the separations coupled to the mass spectrometer still present considerable barriers to accessing the full information content in biological samples. Biomolecule separations are challenging because they present a variety of interaction modalities ranging from hydrophobic to polar to electrostatic. The proposed work addresses the urgent need for improved technologies for novel and more universal separation strategies for biopolymers (e.g., polypeptides, proteins, oligonucleotides) while simultaneously enabling characterization of solute/ionic liquid interactions. The objective of the proposed work is to integrate the emerging field of ionic liquids into novel separation strategies for biomolecular separations with the goal of providing a rapid method for assessing ionic liquids/target analyte interactions a new class of stationary phases enabling separations of complex biomolecular mixtures. We hypothesize that the proposed selectors will provide multimodal retention for the separation of a wide variety of biomolecular analytes. Two specific aims will address this hypothesis: 1. CE Studies of Ionic Liquid Selectors. Capillary electrophoresis will be used to rapidly screen for ionic liquids/selected analyte interactions. We will relate migration data to ionic liquid/analyte affinity. 2. LC Studies of Ionic Liquid Selectors. Retention data will be obtained on the proposed HPLC sorbents beginning systematically with small molecules (e.g., aromatics, nucleotides, amino acids) and extending to larger molecules (e.g., peptides, polynucleotides) as the separation space encompassed by this new media is mapped out.